抗原设计

Chemicals and equipmentsActin Buffer ( 20 mM KPO4 10 mM PIPES 5 mM EGTA 2 mM MgCl2 pH 6.8) Assay buffer (Actin Buffer with 3.7 formaldehyde 0.1 triton 200X TRITC- Phaloidin from 50 mM stock.) DB Prep ...

The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample whether it is from the serum of an immunized animal or the cell supernatant from growing h ...

The sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites capable of binding to antibody since at least ...

OUTLINEClodronate (dichloromethylene diphosphonate) is used for treatment of osteolytic diseases and osteoporosis Its effect is due to inhibittion of the function of osteoclasts. This drug is also tak ...

OUTLINEClodronate (dichloromethylene diphosphonate) is used for treatment of osteolytic diseases and osteoporosis Its effect is due to inhibittion of the function of osteoclasts. This drug is also tak ...

MATERIALS10ml sterile syringes (one for each mouse) 20 gauge needles (one for each mouse) Phosphate Buffered Saline pH 7.2: PBS 1X (GibcoBRL Cat. No. 20012-027) Isoflurane for anesthetizing 70 Ethano ...

This protocol is used in our lab to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protocol contains 40-70 monocytes. This cell suspension is than use ...

OUTLINEThis modification of peritoneal macrophage preparation is designed for a subsequent thrombophagocytosis test. PROTOCOLSacrifice a mouse (use dry ice CO2) Sink a mouse in the 5 water solution o ...

Cut sections at 4µm use a clean water bath with distilled water and let the sections dry upright in order to facilitate adhesion between the section and the charged glass surface. (Slides: Fisher Supe ...

Cut sections at 4µm use a clean water bath with distilled water and let the sections dry upright in order to facilitate adhesion between the section and the charged glass surface. (Slides: Fisher Supe ...

Source of unwanted staining besides poor knowledge of the antibody reactivity and malice is due to: Endogenous enzymes or fluorochromes. Endogenous biotin. Endogenous antibody binding activity (Fc rec ...

Introduction: This method for the detection of cellular proliferation includes several modifications of a previously published protocol (Hayashi et al. 1988 J. Histochem. Cytochem. 36:511-514). This m ...

VisionTo provide scientific collaboration of excellence to National Toxicology Program (NTP) (http://ntp.niehs.nih.gov/) interdisciplinary research programs and DIR research using new technologies in ...

Rationale: The use of DNAse to improve nuclear antigen staining has been published long before the AR era 1 2.DNAse treatment is currently suggested as an unmasking technique for incorporated BrdU nuc ...

Preparation: Cytological PreparationsFixation: Air dry films or cytospin preparations overnight at room temperature. For frozen and paraffin sections refer to the respective ''indirect'' techniques. T ...

Preparation: Cytological PreparationsFixation: Air dry films or cytospin preparations overnight at room temperature. For frozen and paraffin sections refer to the respective ''indirect'' techniques. T ...

You will need the following reagents:- Antibodies Normal Swine Serum (NSS) 1:5 in Tris buffer. 1°: antibody diluted in 1/20 NSS/Tris. 2° antibody diluted in 1/20 NSS/Tris. Streptavidin/biotin complex ...

1. Dissect brains in Drosophila Ringers solution. 2. Fix 20'' (1hr max) in a 0.5ml microfuge tube with 5 formaldehyde-PBS on ice. 3. Rinse 2-3X with PBS (carefully remove solutions with drawn out pipe ...

1. Dissect brains in Drosophila Ringers solution. 2. Fix 20'' (1hr max) in a 0.5ml microfuge tube with 5 formaldehyde-PBS on ice. 3. Rinse 2-3X with PBS (carefully remove solutions with drawn out pipe ...

OverviewR&D Systems provides monoclonal polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed and optimized by R&D Systems'' Immunohistochemical ...