抗原设计

CHIFF preparationDevelop cells for at least 3 hrs to minimize protease activity. Cell are resuspended in DB to 2-4 x 107/ml and shaken at 120 rpm at 22o for 3-6 hrs. Cells were harvested and washed wi ...

Sandwich ELISAThe sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites capable of binding to antibody s ...

MaterialsNUNC-Immuno Plate IIF. Horseradish Peroxidase-Streptavidin (Zymed; 43-4323) PBS or Balanced Salt Solution (BSS). Do not use RPMI or any medium that contains biotin. BSA (1% in PBS). ABTS subs ...

ELISA (Enzyme-Linked ImmunoadSorbent Assay)You will need the following solutions: PBS (Phosphate-Buffered Saline Ca/Mg free) :- Solute1x10xNaCl8g80gKCl0.2g2gNa2PO4 (anhydrous)1.15g11.5gKH2PO4 (anhydro ...

Gangliosides ELISA protocolAuthor: pingsunjimSource: Contributed by PingsunjimAbstract: This protocol can be used for detection of gangliosides.Spicific antibodies to gangliosides are added to cells a ...

Indirect ELISAThe indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample whether it is from the serum of an immunized animal or the cell supernatant ...

Live Cell ELISA ProtocolAuthor: Nanci DonackiSource: Contributed by Nanci Donacki ReagentsDMEM:10% FBSDMEM:10% FBS:1% HEPES (DFH)10X Substrate Buffer pH 6.036.6 g Citric Acid monohydrate113.5 g ...

Peptide Inhibition ELISA ProtocolAuthor: Nanci DonackiSource: Contributed by Nanci DonackiReagents PBSPBS:0.1% BSA:0.05% Tween 20 (PBT)PBS:2% BSA10X Substrate Buffer pH 6.036.6 g Citric Acid mon ...

Periodate Treatment of Fixed Cell PlatesAuthor: Nanci DonackiSource: Contributed by Nanci DonackiReagentsSodium Borohydride (NaBH4) - 1 mg/mlCarbonate:Bicarbonate Buffer pH 9.6 Sodium Periodate (NaIO4 ...

FIXATION WITH FORMALDEHYDE-ACETONEMaterials1. Dulbecco's PBS 37oC. 2. Fixation buffer i.e. PBS PHEM or modified Hanks ("cytoskeleton buffer") 1.33x. 3. Formaldehyde 16% EM Sciences. Store at room temp ...

Fixation with GlutaraldehydeMaterials vol (for 500 ml) of stock mg 1. 137 mM NaCl 34.25 ml 2 M 5 mM ...

Embedding in Resins for ImmunocytochemistryBiological specimens are embedded in resin so that they can be thin sectioned. Many different resin formulas are currently available and all have been used f ...

Freeze Substitution How to prepare samples using freeze-substitution without an expensive machine.This method has been used successfully in our laboratory since 1994. The protocol assumes all users wi ...

IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Diaminobenzadine (DAB) brown detectionSections of paraformaldehyde fixed OCT-embedded vascular tissue are secti ...

MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems 'AF' or 'BAF' series) or selected monoclonal antibodies.Secondary An ...

IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Vector Red DetectionJosiah N. Wilcox and Romesh R. Subramanian - Emory University - September 20 1993 Sections ...

Immunostaining Protocol (now with demo videos/stills)We recommend that you first download and read the PDF file containing the step-by-step protocol and solution recipes. Using that as your guide you ...

Immunostaining of Cells Adherent to CoverslipsProcedure:1) Immerse 18 mm2 glass coverslips in EtOH. In the tissue culture hood individually pull out and carefully flame to sterilize. Allow to cool the ...

Introduction to ImmunocytochemistryImmunocytochemistry is the name given to methods that use antibodies to detect the location of proteins within cells. The antibodies bind specifically to the protein ...

General remarks:Labeled sections have to be fixed to avoid loss of label during the enhancement procedure and thoroughly washed with bi-distilled water to remove ions (especially chloride ions) which ...