植物生理

Methods for in vitro initiation and multiplication and general culture practices of Rhododendron are presented. Also acclimatization procedures are described. Protocols for callus, shoot, and root induction are described. Several protocols for breeding applications are hi ...

Numerous shoots were directly regenerated from the leaf explants of Lysionotus pauciflorus on the MS medium containing 0.5–2 �M NAA with or without 1 �M BA. The calli were induced from the leaves on MS medium supplemented with 2 �M 2, 4-D. The calli proliferated about four times in fresh weight in the liquid m ...

With more than 450 species, Passiflora is the most important genus of the family Passifloraceae. It comprises many species grown for their edible fruits, for their high ornamental value, and further for the therapeutic properties. With their striking exotic flowers, they are of particular i ...

Chrysanthemum flowers Chrysanthemum x grandiflorum (Ramat.) Kitam., are commercially significant worldwide as there are large number of cultivars for cut flowers, pot flowers, and garden flowers. Commercial in vitro multiplication of chrysanthemum is often based on stem nodal ex ...

In vitro propagation of rose is an important tool for rapid multiplication and development of new cultivars with desirable traits. However, successful in vitro propagation requires an understanding of specific requirements and precise manipulation of various factors. Efficie ...

Codiaeum variegatum (L) Blume cv. “Corazon de oro” and cv. “Norma” are successfully micropropagated when culture are initiated with explants taken from newly sprouted shoots. The establishment and multiplication steps are possible when 1 mg/L BA or 1 mg/L IAA and 3 mg/L 2iP are added to MS medium, acco ...

Geraniums (Pelargonium spp.) are among the most popular bedding and pot plants (25 % of the French domestic market). On one hand, as vegetatively propagated plants, Pelargonium are submitted to pathogen pressure. On the other hand, innovation via interspecific hybridisation faces some di ...

Tissue culture techniques are routinely used for mass propagation and the establishment of disease free stock material. Virtually all pot type Anthuriums available in the market today are produced by tissue culture. In this chapter, we describe an efficient protocol to obtain Anthurium ...

Plants respond and adapt to drought, cold, and high-salinity stresses. Stress-inducible gene products function in the stress response and tolerance in plants. Using cDNA microarrays and oligonucleotide microarrays, stress-inducible genes have been identified in various plant ...

A powerful means to learn about gene functions in a developmental or physiological context in an organism is to isolate the corresponding mutants with altered phenotypes. Diverse mutagenic agents, including chemical and biological, have been widely employed, and each comes with its own a ...

MicroRNAs (miRNAs) are approximately 22-nucleotide (nt)-long non-coding RNAs that play a key role in plant development and abiotic stresses. We have developed a simple but effective array platform for profiling plant miRNAs from various plant species. The array is composed of 188 non-red ...

In plants, small RNA (microRNAs and other endogenous small RNAs)-guided target gene expression is vital for a wide variety of biological processes including adaptation to stress conditions. Identification of stress-regulated microRNAs or other classes of endogenous small RNAs a ...

Abiotic stresses often cause metabolic imbalances which affect cellular redox homeostasis and alter the rate of reduction state of functional and regulatory protein thiols and the rate of reactive oxygen species release. Excessive displacement from redox equilibrium causes ox ...

Among the open-ended techniques for identifying differentially expressed genes in response to stress, the PCR-based suppression subtraction hybridization (SSH) is widely used. The popularity of this technique stems from the ease of conducting this procedure in any laboratory set ...

Reactive oxygen species (ROS) are formed in biological systems as part of normal metabolism. Adverse environmental factors like drought stress result in increased levels of ROS that are detrimental to the plant (1, 2). To avoid damage caused by these excess ROS, plants have developed elaborate ...

Thin cell layers or TCLs have been a fundamental corner-stone of tissue culture of all plant species, including floricultural and ornamental plants (FOPs). In this review, the current status of the use of TCL technology to specific FOPs will be outlined while certain successes and difficulti ...

Aldehydes are highly reactive and ubiquitous molecules involved in numerous biochemical processes and physiological responses. Many biologically important aldehydes are metabolized by the superfamily of NAD(P)+-dependent aldehyde dehydrogenases . Here we describe a st ...

Two-dimensional electrophoresis is a powerful tool to explore the plant proteome and to unravel changes in protein expression between samples. However, the acquisition of images on which thousands of spots may be resolved has some weak points, as always pointed out by scientists working wi ...

In response to water-deficit stress, plants alter expression of thousands of genes and as a result, cellular, physiological, and biochemical processes are modified. Understanding the functional role of water-deficit stress-responsive genes is important in order to develop stres ...

The regulation of gene activity is a crucial factor in coordinating development, growth and acclimation to environmental changes. By this means, metabolic processes are adjusted according to cellular needs by changing gene expression patterns. In the genome of the model plant Arabido ...