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Rubber Tree (Hevea brasiliensis Muell. Arg)

Rubber tree (Hevea brasiliensis Muell. Arg.) is an important industrial crop for natural rubber production. At present, more than 9.5 million hectares in about 40 countries are devoted to rubber tree cultivation with a production about 6.5 million tons of dry rubber each year. The world supply of na ...

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Banana (Musa sp.)

Cultivated bananas are vegetatively propagating herbs, which are difficult to breed because of widespread male and female sterility. As a complementary gene transfer method in banana, the described Agrobacterium protocol relies on highly regenerable embryogenic cell cultur ...

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Citrus

Since the initial reports on production of transgenic Citrus via Agrobacterium-mediated transformation, significant progress has been made, and many steps of this procedure using the juvenile tissue explants have been improved. Abundant availability of starting material and r ...

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Coffee (Coffea sp.)

Coffee (Coffea sp.) is a perennial plant widely cultivated in many tropical countries. It is a cash crop for millions of small farmers in these areas. As compared with other tree species, coffee has long breeding cycles that make conventional breeding programs time consuming. For that matter, gen ...

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Papaya (Carica papaya L.)

Transgenic papaya plants were initially obtained using particle bombardment, a method having poor efficiency in producing intact, single-copy insertion of transgenes. Single-copy gene insertion was improved using Agrobacterium tumefaciens. With progress being made in gen ...

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Sugarcane (Saccharum spp.)

We describe the procedures for recovering transgenic sugarcane from co-cultivation of both calli and in vitro plants with Agrobacterium tumefaciens. The correct tissue culture strategies and the use of super-binary vector or super-virulent strain are crucial for the successful su ...

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Pineapple [Ananas comosus (L.) Merr.]

A procedure for pineapple genetic transformation is described, which involves temporary immersion bioreactors (TIB) for selection of transgenic plants. Success in the production of transgenic pineapple plants combines tissue culture factors. Firstly, the use of regenerable ...

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American Chestnut [Castanea dentata (Marsh.) Borkh.]

The key to successful transformation of American chestnut is having the correct combination of explant tissue, selectable and scorable markers, and a reliable regeneration system. Rapidly dividing somatic embryos, growing as proembryogenic masses, are a suitable tissue; the bar ge ...

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Apple (Malus domestica)

Apple (Malus � domestica) is one of the most consumed fruit crops in the world. The major production areas are the temperate regions, however, because of its excellent storage capacity it is transported to distant markets covering the four corners of the earth. Transformation is a key to sustaining ...

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Blueberry (Vaccinium corymbosum L.)

Recent advances in plant biotechnology have led to a reliable and reproductive method for genetic transformation of blueberry. These efforts built on previous attempts at transient and stable transformation of blueberry that demonstrated the potential of Agrobacterium tumef ...

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Grapevine (Vitis vinifera L.)

Grapevine (Vitis) is considered to be one of the major fruit crops in the world based on hectares cultivated and economic value. Grapes are used not only for wine but also for fresh fruit, dried fruit, and juice production. Wine is by far the major product of grapes and the focus of this chapter is on wine grape cult ...

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Strawberry (Fragaria ananassa)

Genetic transformation in strawberry (Fragaria spp.) can be achieved by using the Agrobacterium-mediated procedure on leaves from in vitro proliferated shoots. Regardless of the sufficient regeneration levels achieved from leaf explants of some commercial strawberry genot ...

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Walnut (Juglans)

Walnut species are important nut and timber producers in temperate regions of Europe, Asia, South America, and North America. Trees can be impacted by Phytophthora, crown gall, nematodes, and cherry leaf roll virus; nuts can be severely damaged by codling moth and Xanthomonas blight. The long-g ...

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Carnation (Dianthus caryophylus L.)

Carnation is a valuable crop for the cut flower industry and demand for new and improved varieties is growing. However, genetic transformation of carnations is currently limited because of a lack of efficient routine technique. In this chapter, we present an easy and effective protocol for gene ...

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Chrysanthemum (Dendranthema grandiflora)

This chapter provides reproducible methods for the transformation of Dendranthema � grandiflora ‘Shuhou-no-chikara’ (standard) and ‘Lineker’ (spray) stem internode thin cell layers (TCLs) and conventional stem internode explants using Agrobacterium strains carrying a b ...

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Orchids (Cymbidium spp., Oncidium, and Phalaenopsis)

Recent advances in genetic engineering have made the transformation and regeneration of plants into a powerful tool for orchid improvement. This chapter presents a simple and reproducible Agrobacterium tumefaciens-mediated transformation protocol and molecular screen ...

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Petunia (Petunia hybrida)

Petunia hybrida genetic transformation continues to be a valuable tool for genetic research into biochemical pathways and gene expression, as well as generating commercial products with varying floral colors. In this chapter, we describe a simple and reproducible genetic transfo ...

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Rose (Rosa hybrida L.)

Although rose transformation is successful, it remains difficult to transform myriad rose species as well as different rose genotypes. In this protocol, a detailed description of rose transformation is presented. This protocol relied on Agrobacterium-mediated transfer of embr ...

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Ginseng (Panax ginseng)

Genetic transformation is an attractive way to improve Panax ginseng C. A. Meyer by introducing new genetic materials and altering metabolic pathways that regulating the production of secondary compounds. In P. ginseng, production of transgenic plants via Agrobacterium tumefaci ...

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Hemp (Cannabis sativa L.)

Hemp (Cannabis sativa L.) suspension culture cells were transformed with Agrobacterium tumefaciens strain EHA101 carrying the binary plasmid pNOV3635. The plasmid contains a phosphomannose isomerase (PMI) selectable marker gene. Cells transformed with PMI are capable of met ...

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