常用设备/其他

This is prepared as a 1.2% solution and used in mice at a dosage rate of 0.2 ml/10grams body weight which approximates 240 mg/kg. This compound is subject to photodegradation and should be stored at 4 ...

The method we use is based on work of Dr. Hazel Cheng at the University of Toronto and works for both colon and the small intestine.First we excise the whole colon from cecum to rectum. This is easily ...

Prior to the initiation of experiment:Obtain IACUC approval Sterilize surgical instruments and Q-tips I. Harvest Donor TissueEuthanize donor mouse (in accordance with institution's SOP) Wet hair back ...

PROTOCOLGeneral anesthesiainject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation of ...

What is Anesthesia?Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief) amnesia (loss of memory) and immobilization. The drugs ...

1) Transfer to a 24 wells plate the desired colonies.2) Once the cells are attached (at least 8 hours after tripsinizing them) add ~1 ml ofDME (met- cys-).3) Add 3l of Met 35S Cys 35S trans label.4) I ...

Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipment (PPE). Many of them are common sense and apply to all la ...

Although many protocols for tubulin preparation are available the procedure described below is the simplest and highest yielding preparation I have done. The protocol calls for 3 pig brains and shoul ...

Tubulin is purified from bovine/porcine brain by two cycles of polymerization/depolymerization followed by removal of copurifying proteins on a phosphocellulose (PC) column. The procedure described he ...

N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide. Decontamination of solutions 0.5mg/mlThis method reduc ...

The single most important advance in the use of centrifugal force to separate biologically important substances. was the coupling of mechanics optics and mathematics by T. Svedberg and J.W. Williams i ...

Reaction volume 100 ul5 ul NADPH (100 mM)x ul cell extract so you get 50 % conversion0.2 uM C14-Testosteroneadd Tris-Citrate Buffer pH 6 to an endvolume of 100 ulmix C14-Testosterone and Citrate Buffe ...

Radioactive DecayIsotopes of a given element have nuclei with the same number of protons but different numbers of neutrons. Some isotopes are stable however radioisotopes are unstable and disintegrate ...

With the aid of spectroscopy the quantitative analysis of nucleic acids and proteins has established itself as a routine method in many laboratories. It includes absorption measurements in the ultravi ...

Figure G.1 Electromagnetic Radiation Spectrum Figure G.2. Schematic light transmission Figure G.3. Use of the Spec 20 A spectrophotometer or colorimeter makes use of the transmission of light through ...

We primarily use the Gilson micropipets in the core course labs. We have four sizes identified by the number on the round button on the plunger. The value is the maximum volume in microliters that can ...

OverviewGlycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors the main disadva ...

Alkali DenaturationAlkali Denaturation of Supercoiled Plasmid DNA 1) To 20 ul of purified concentrated DNA solution add 2 ul of freshly prepared NaOH/EDTA solution and incubate at 37 degrees for 30 mi ...

Purpose: To break up high molecular weight human placental DNA into fragment sizes of 500 bp or less which can be used as competitor DNA in Southern and in situ hybridizations. This method can also be ...

We regularly reuse our large 25x25 cm plating trays; initially however we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory ...