常用设备/其他

（一）小鼠用右手提起小鼠尾部，放在实验台上，当其向前爬行时，左手抓住两耳及头颈部皮肤，再置小鼠于左手心，拉直四肢并用手指夹住肢体固定。右手可行注射或其它操作。小鼠固定器及固定器固定法（二）大鼠右手轻轻抓住大鼠尾部向后轻拉，左手抓紧鼠两耳及头颈部皮肤，并将其固定在左手中，右手可行注射或其它操作。大鼠固定器及固定器固定法（三）兔1、正确的抓取兔的方法如下：说明：1、可伤两肾；2、可造成皮下出血；3、 ...

对于像黑色、棕色小鼠以及裸鼠，我们不能单纯用常规方法编号，因此对于这类动物，可以采用一些特殊的编号方法。一、采取耳朵打孔编号1号双耳无孔；　　2号左耳打孔；　　3号右耳打孔；　　4号双耳打孔；　　5号左耳双孔。二、耳缺法左前缺-1号；　　左后缺-2号；　　右前缺-3号；　　右后缺-4号；　　1+2――5号；　　3+4――6号；　　1+3――7号；　　2+4――8号。耳缺法隔着笼子也好观察。裸鼠耳朵 ...

捉拿和固定是动物实验操作技术中最基本最简单而又很重要的一项基本功。 以下内容需要登录后才能完全查看，请您登录马上登陆| 企业会员注册 ...

分子生物学常用溶液配制一、分子生物学常用贮存液的配制1．30%丙烯酰胺溶液【配制方法】将29g丙烯酰胺和1g N，N’-亚甲双丙烯酰胺溶于总体积为60ml的水中。加热至37℃溶解之，补加水至终体积为100ml。用滤器（0.45μm孔径）过滤除菌，查证该溶液的pH值应不大于7.0，置棕色瓶中保存于室温。【注意】丙烯酰胺具有很强的神经毒性并可以通过皮肤吸收，其作用具累积性。称量丙烯酰胺和亚甲双丙烯酰胺 ...

General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa. Experimental surgery has great potential for causing pain or distress to an ...

INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic procedures. This includes the use of surgical gloves ma ...

TISSUES TO BE PROCURED(minimally and preferably within 5-8 hours after death):1. Brain2. Liver3. Muscle4. Skin5: Others as the specific case dictates PROCESSING AND STORAGE OF TISSUES:1. BrainRemo ...

ProcedureAfter deep ether anesthesia dissect the rat’s liver (Wistar albino rats 200 � 250 g) by cutting on the ventral side. Fix 2 � 3 mm. of the liver tissue in neutral buffered formalin (10% formal ...

Administration of Fluids and Drugs (Overview):Gastro-Intestinal Tract:Oral or per os (po)- - through the mouth Gavage - - into the stomach via a feeding needle Parenteral Administration:General Consid ...

Materials Needed: Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip 2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samplesRack to hold tubesFine tip marker to label tu ...

The ear veinsThe marginal ear veins are the only veins that are easily visible on pigs of any size. Usually there are three prominent veins. The lateral or central vein is usually the largest of these ...

Mice are injected by an intraperitoneal route with a Luciferin solution (15 mg/mL or 30 mg/kg in PBS dose of 150 mg/kg) that is allowed to distribute in awake animals for about 5-15 minutes. The mice ...

Preparation of Islets for Transplant (Tx)Under an inverted microscope hand-pick islets using a P200 pipetman and straight pipet tip from the cultured islets in a 100mm plate. Count islets 100 at a tim ...

Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in human hematopathology. A long tradition in experimenta ...

You need to buy glass slides with frosted sandblasted ends (Fisher Scientific Catalog N. 12-552). Frosting by painting (e.g.Superfrost) should not be used. Pre-clean the slides by rubbing the edges o ...

Prepare a 10ml syringe fitted with a 26G short needle and filled with 5 to 7 ml of medium and 2 to 3 ml of air. Air is important. Prepare a Pasteur pipette with rubber pipetter. Euthanize by CO2 inha ...

if mouse is found dead if mouse is a newborn or If mouse is alive or moribund and above one week of age Routine dissection Formalin fixation Storage of smears and slides Tissue embedding in paraffin ...

Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help ensure optimal results for aseptic survival surgery ...

Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the Guide for the Care and Use of Laboratory Animals all survi ...

Isolation of bone marrow (contributed by Chris Jackson (chris.jackson@bris.ac.uk)) A protocol I have used to isolate rat bone marrow is: 1. Kill the rat and dissect out the femurs cutting the bones cl ...