常用设备/其他

1. Simple Dilution (Dilution Factor Method)A simple dilution is one in which a unit volume of a liquid material of interest is combined with an appropriate volume of a solvent liquid to achieve the de ...

08-15 Buffer CompoundMWFinal concentration200 mlGlycine75.070.1 M1.5 g1 M MgCl210 mM2 mlKOHto pH 9H2Oto 200 mlA Acetic Acid 1 M CompoundMWFinal concentration50 mlAcetic Acid 98% (~17 M)1 M3 ml ...

AcO---- part one ---(AcO)2Ca 1; 1.5MAcOK 1; 2; 3; 4; 5; 6; 7; 8M(AcO)2Mg 1MAcONa 1; 2; 3; 4MpH of Ac K/Na solutionsAcONH4 1; 2; 3; 4MCO3-K2CO3 1; 2; 3; 4; 5; 6MKHCO3 1MNa2CO3 1; 2MNaHCO3 1M( ...

Organic substances.pKa and temperature dependence of pH for common buffers.ATP 0.1MBetaine 5MCresol red (Na) 50mMDTT 1M 2.2MdNTP’s 100mMEDTA 0.5MEtBr 10mg/mlGelatin 2%Glucose 1; 1.5; 2MGuanidine HCl 1 ...

The following is a list of the solutions and chemicals required throughout the laboratory manual. It is organized alphabetically and individual exercises list the materials needed for that exercise. F ...

Turn on machine and wait for it to warm up Wash glass cuvettes thoroughly before use and if 2 are used then make sure they are a ‘matching’ pair. For Spec. in Dave Andersons bay then press ‘goto ?’ ty ...

The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue to protein.There is no interference from cations ...

Introduction The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change in absorbance in Coomassie Blue G-250 upon binding o ...

The Lowry procedure is one of the most venerable and widely-used protein assays being first described in 1951 J. Biol. Chem. 193: 265-275 (1951). Under alkaline conditions copper complexes with prote ...

(contributed by Chris Jackson (chris.jackson@bris.ac.uk)) A protocol I have used to isolate rat bone marrow is: 1. Kill the rat and dissect out the femurs cutting the bones close to the chondyles. Kee ...

The method we use is based on work of Dr. Hazel Cheng at the University of Toronto and works for both colon and the small intestine.First we excise the whole colon from cecum to rectum. This is easily ...

OUTLINEPROTOCOLGeneral anesthesia1. inject i.p. 250µl/mouse of the ANESTHETIC mixture 2. for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet)Pre ...

Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in human hematopathology. A long tradition in experimenta ...

在PCHOME数码影象俱乐部论坛中，有关光学变焦和数码变焦的问题已经被网友们提出N多次了。大家对此问题都好象有了结论，没有什么可以再讨论的了。但仔细考虑，其中仍有不少有趣的东西值得我们去想一想。因此，我想在下面的文章里细说一下数码变焦以及与其相关的一些问题。让我们先看一个最简单的数学问题。下图是一个直角三角形，学过数学的朋友都知道，角A的大小是与两条直角边a和b有关的。直角边a越长则角A越大，而直 ...

　　传统胶片的成象原理的基本过程是：涂有溴化银晶体的感光层受光后结构变化产生潜影，通过化学方法显影工艺使潜影影象得到固定，其操作工艺复杂费时而且稳定性易受化学药剂的温度等诸多因素的影响。而数字相机将这一复杂过程用电荷偶合器件CCD通过电子物理方式在一瞬间完成。CCD是表面按一定排列顺序布满硅点的芯片，每个硅点相当于普通胶片中的溴化银颗粒，不同的是CCD芯片点阵的分布是按BGB（红、绿、蓝）三个基 ...

纪念Rudolph博士于1902年设计的Carl Zeiss Tessar生日快乐，Tesser！1902年4月25日，柏林的皇家专利委员会将编号为142294的专利证书颁发给了Carl Zeiss Jena公司生产的以Tesser命名的镜头。自此一个辉煌的镜头家族开始逐渐发展壮大起来。当我们将目光转向光学发展史的开端，我们就会看到，在光学历史的早期(即1839-1855/60年的达盖而时期)，市 ...

Pentax的世界第一Xitek编译 2001年10月1日年代机型理 由1952Asahiflex生产了日本第一台SLR；1954Asahiflex II生产了世界上第一台具有瞬回反光镜系统的照相机；1957Asahi Pentax第一次将五棱镜用于SLR的取景器；1964Spotmatic生产了世界上第一台具有TTL测光系统的SLR；1966　Asahi生产的照相机数量达到第一个一百万台( ...

Fujica早期35mm照相机Fujica系列SLR已经停产多年，许多机型已经不为人所知。这里汇集了一些Fujica SLR和镜头的一些资料。 1、早期的Fujicarex照相机型号推出时间快门速度范围电池说明Fujicarex19621-1/500+B不用非TTL测光，镜间快门Fujicarex II19631-1/500+B不用非TTL测光，镜间快门，在对焦屏上旁轴取景Fujicared I ...

Konica单反相机简史第一部柯尼卡单反相机是1960年代Konica F。它有一部直走幕廉快门，Hi-Synchro，最高速度为1/2000秒，闪光同步为1/125 秒。在当时为最快。想想直到七十年代，最快的专业相机也不过是1/1000秒而已。但是因为这个快门制造麻烦，而且太出色了，没有其它的制造商跟进。( 柯尼卡从1953年开始花了7年来研制这部快门，另外从1955年开始花5年研制机身。)碰 ...