显微镜

SECTIONS4micron paraffin wax sectionsSOLUTIONS1. Alcian Blue pH 2.5Alcian Blue 1g3% Acetic Acid 100ml2. 1% Periodic Acid 3. Schiffs ReagentMETHODTake sections to waterPlace in Alcian Blue for 5 minute ...

Procedure:Incubate sections in the incubating medium at room temperature for 1 - 3 hours. Two hours is sufficient in most cases. Wash in distilled water for 2 minutes. Counterstain with Nuclear Fast R ...

Incubate sections in the incubating medium at 37°C for 5 - 12 hours. Long incubation periods are needed to get significantly visible reaction product. Wash in distilled water for 2 minutes. Countersta ...

Embedding in plastic provides many advantages to the histotechnologist. Thinner sections can be made providing improved detail. Better support is given to cellular components offering improved morphol ...

Embedding in a plastic often supplies the support necessary to successfully section various materials samples especially porousand inhomogeous samples. GMA has been used to embed and section polymer r ...

Procedure: Oxidize sections in 0.4% periodic acid for 30 minutes at 57°C. Wash in running tap water or sodium sulfite solution (to avoid a general pink background). Rinse in three times in distilled w ...

SectioningSectioning is best done with a rotary or sledge microtome such as the JB-4 Microtome. The features to look for are retraction of the specimen on the return stroke and motorized motion of the ...

Procedure: Dry the sections for 2 hours at 37°C on a slide warmer. This must be done whether the slides have been stored at 4°C for some time or just collected. Pretreat the sections with trypsin at 3 ...

Day 1 Step 1: Read and understand the Material Safety Data Sheets associated with each of the chemicals used in this procedure. Use of rubber gloves lab coat and safety glasses is mandatory.Step 2: On ...

IntroductionSpecimens that are to be examined in the transmission electron microscope (TEM) usually have to be thin dry and contain contrast (usually from a heavy metal stain. One of the easiest ways ...

Basic Processing Protocol for Tissue Culture Cells Grown in Plastic Dishes. This method can easily be applied most biological samples without problem. 1). Wash cells with PBS twice. Remove the PBS and ...

Basic Processing Protocol for Tissue Culture Cells Grown in Plastic Dishes. This method can easily be applied most biological samples without problem. 1). Wash cells with PBS twice. Remove the PBS and ...

A few notes on fixation:The usual fixative for paraffin embedded tissues is neutral buffered formalin (NBF). This is equivalent to 4% paraformaldehyde in a buffered solution plus a preservative (metha ...

Deparafinization: The paraffin is removed from the section with xylene. If the tissues are to be stained with an aqueuous solution then the slides must rehydrated in graded ethanol baths. Unless a tim ...

ReagentsStains (change after every 3rd use) Harris hematoxylin: 1X stock. Anatech Lmtd. Cat #842. (616) 964-6450 Gill's Hematoxylin: See recipe. Eosin: 1x stock. Anatech Cat #837. Solutions (make fre ...

ReagentsStains (change after every 3rd use) Harris hematoxylin: 1X stock. Anatech Lmtd. Cat #842. (616) 964-6450 Gill's Hematoxylin: See recipe. Eosin: 1x stock. Anatech Cat #837. Solutions (make fre ...

Syringes 1 mL with 36G needlesBrdU: 3-10 mg/mL in PBSFixative: Methyl Carnoy's or 4% paraformaldehyde in PBSParaffin processing materialsOrganic solvents: Xylene EtOH MeOH0.3% H2O2: 2 mL 30% H2O2 i ...

MaterialsFixative2% Paraformaldehyde (in PBS)Staining solution5 mM potassium ferricyanide 5 mM potassium ferrocyanide 2 mM MgCI 0.01% sodium deoxycholate0.02% Nonidet P-40 (NP-40) in PBS. X-gal Stock4 ...

MaterialsCold Acetone (-20ºC) in Coplin jarsPBS BenchKote paper plastic lidsAnti-digoxigenin-HRP diluent: 1% BSA in PBS (store 10 mL aliquots of BSA at -20ºC)Anti-rat-digoxigenin diluent: 5% Milk po ...

MaterialsAnti-digoxigenin-AP conjugate (Roche #11093274910 150U/200 uL $168)Levamisole (Sigma #T1512 2 g $10) (blocks endogenous phosphatase activity)BCIP (Roche #11383221001 3 mL $49)NBT (Roche #113 ...