基因组学

Optimal arbitrary primer length for Differential DisplayThe concept of differential display is to use a limited number of short arbitrary primers in combination with the anchored oligo-dT primers to ...

Optimal arbitrary primer length for Differential DisplayThe concept of differential display is to use a limited number of short arbitrary primers in combination with the anchored oligo-dT primers to ...

Reagents:ChIP sonication Buffer (1% Triton X-100 0.1% Deoxycholate 50 mM Tris 8.1 150 mM NaCl 5 mM EDTA):10 ml 10% Triton X-1001 ml 10% Deoxycholate5 ml 1 M Tris-Cl pH 8.11 ml 0.5 M EDTA3 ml 5 M NaCl ...

CHIP PROTOCOL FOR YEASTCHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEASTThis protocol is derived from a paper by Miriam Braunstein and is based on work in the Allis lab. The procedure was writte ...

Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab) This protocol is based upon protocols from Mark Biggin Dave Allis and Richard Treisman plus a fair amount of trial and error. ...

Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab) This protocol is based upon protocols from Mark Biggin Dave Allis and Richard Treisman plus a fair amount of trial and error. ...

Chromatin IP (CHIP assay)This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. Gen & Dev 11 p83-93 and was obtained from Flick K. (The Scripps Research Ins ...

Chromatin IP (CHIP assay)This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. Gen & Dev 11 p83-93 and was obtained from Flick K. (The Scripps Research Ins ...

CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEASTCHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST2 Days Before--Start 5 mL overnight from single colony.1 Day Before--Subculture overnight ...

Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)Day 11. Add formaldehyde directly to tissue culture media to a final concentration of 1%. We generally use 1 x 107 cells per anti ...

Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)Day 11. Add formaldehyde directly to tissue culture media to a final concentration of 1%. We generally use 1 x 107 cells per anti ...

Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab) This protocol is based upon protocols from Mark Biggin Dave Allis and Richard Treisman plus a fair amount of trial an ...

Mannie Liscum(1) and Paul Oeller(2)Department of Plant BiologyCarnegie Institution of WashingtonStanford CA 94305(1)Current address:http://www.biosci.missouri.edu/liscum/LiscumLabPage.html (site conta ...

Mannie Liscum(1) and Paul Oeller(2)Department of Plant BiologyCarnegie Institution of WashingtonStanford CA 94305(1)Current address:http://www.biosci.missouri.edu/liscum/LiscumLabPage.html (site conta ...

RLCS (Restriction Landmark cDNA Scanning) Protocolfor inmature barley grains(probably applicable for other plant materials)RLCS that was developed by Suzuki et al. (1996) displays many cDNA species q ...

RLCS (Restriction Landmark cDNA Scanning) Protocolfor inmature barley grains(probably applicable for other plant materials)RLCS that was developed by Suzuki et al. (1996) displays many cDNA species q ...

Microsatellite markers: isolation with non-radioactive probes and amplification Arnaud Estoup and Julie TurgeonThe main goal of this technical document (63 pages) is to provide geneticians and ecolo ...

b-galactosidase Solution AssayReference: Simon and Lis (1987) NAR 15: 2971-2988.SolutionsCPRG Assay Buffer50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.51 mM MgCl2 0.1 ml 1M MgCl2up to 100 ml with Qstore at r ...

b-galactosidase Solution AssayReference: Simon and Lis (1987) NAR 15: 2971-2988.SolutionsCPRG Assay Buffer50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.51 mM MgCl2 0.1 ml 1M MgCl2up to 100 ml with Qstore at r ...

ES injection pipette manufactureThe following is an outline of the method that I use to manufacture injection needles for ES cell injection. I use high quality borosilicate glass obtained from World P ...