This method for the simplified purification of mouse DNA is based upon an article entitled �Simplified mammalian DNA isolation procedure� by Peter W. Laird from Nuclei Acids Research Vol. 19 No. 15 pa ...
General precautions to be followed during handling of any genomic DNA sample: Avoid Shearing of the DNA by limiting mixing to inversion and gentle shaking. Vortexing of samples is not required to perf ...
1. Cut 0.5 cm of tail and mark mouse ears or toes2. Incubate at 55°C O/N in:500 l Tail Extraction Buffer 10 l Proteinase K (10 mg/ml)12.5 l 20% SDS 3. Add 150 l 5 M NaCl Vortex 5 minMicrofuge 15 min ...
1. Cut 0.5 cm of tail and mark mouse ears or toes2. Incubate at 55°C O/N in:500 l Tail Extraction Buffer 10 l Proteinase K (10 mg/ml)12.5 l 20% SDS 3. Add 150 l 5 M NaCl Vortex 5 minMicrofuge 15 min ...
The DNEasy Kits From Qiagen work well for preparing DNA from toes and tails. Alternatively the following protocol may be used. Cut the last phalange of one toe into 0.5 ml of lysis buffer (100 mM Tris ...
Although all of the DNA in an eukaryotic cell replicates during the S-phase of cell cycle there is a significant difference in the actual time in S-phase when a given chromosomal segment replicates. M ...
1 For cells cultured in 2D about 1-2X107 S1 cells were growth in 100mm dish and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. ...
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEASTThis protocol is derived from a paper by Miriam Braunstein and is based on work in the Allis lab. The procedure was written by Pam Meluh and upda ...
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEASTThis protocol is derived from a paper by Miriam Braunstein and is based on work in the Allis lab. The procedure was written by Pam Meluh and upda ...
Max Haring1 Sascha Offermann2 Tanja Danker2 Ina Horst2 Christoph Peterhansel2 and Maike Stam1 1 Swammerdam Institute for Life Sciences Universiteit van Amsterdam Kruislaan 318 1098 SM Amsterdam ...
ChIP-Seq_Sample_Prep_11257047_RevA.pdf ...
ChIP Protocol-Myerslab-112607.pdf ...
Preparing Samples for.pdf
Preparing Samples for.pdf
Day 0 Block Staph A cells (Pansorbin CalBiochem 507862) Thaw 1 tube (200 μL) of Staph A cells for approximately 5 experimental ChIPs. Add 25 μL of herring sperm DNA (Promega D1815) 10 mg/ml); previous ...
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in ...
INTRODUCTION Chromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used t ...
INTRODUCTION This protocol describes the use of chromatin immunoprecipitation technology (ChIP) to analyze interactions of proteins or protein complexes with DNA in vivo. In this approach the material ...
INTRODUCTION This protocol describes the use of chromatin immunoprecipitation technology (ChIP) to analyze interactions of proteins or protein complexes with DNA in vivo. In this approach the material ...
INTRODUCTION In cells and tissues the histone proteins that constitute the nucleosomes can present multiple post-translational modifications such as lysine acetylation lysine and arginine methylation ...