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Hydrogels based on peptide self-assembly form an important class of biomaterials that find application in tissue engineering and drug delivery. It is essential to prepare peptides with high purity to achieve batch-to-batch consistency affording hydrogels with reproducible pro ...

Most hubs' binding sites are able to transiently interact with numerous proteins. We focus on β-rich hubs with the goal of inferring features toward design. Since they are able to interact with many partners and association of β-conformations may lead to amyloid fibrils, we ask whether there is some ...

Recent developments in the field of nanobiology have significantly expanded the possibilities for new modalities in the treatment of many diseases, including cancer. Ribonucleic acid (RNA) represents a relatively new molecular material for the development of these biological ...

The formation of a nanostructure by self-assembly of a peptide or protein building block depends on the ability of the building block to spontaneously assemble into an ordered structure. We first describe a protocol of fusing homo-oligomer proteins with a given three-dimensional (3D) str ...

There is an increasing demand on the development of “smart” switchable interfaces since controlling surface topography and chemical functionality on a nanometer scale is crucial for numerous biomedical applications. Those surfaces, which are based on stimuli responsive polym ...

An important goal in the development of highly functional organic materials is to design self-assembling molecules that can reproducibly display chemical signals across length scales. Within the biomedical field, biomolecules are highly attractive candidates to serve as bioa ...

In this chapter, we describe two methods for bio-producing recombinant repetitive polypeptide polymers for use in biomedical devices. These polymers, known as elastin-like recombinamers (ELRs), are derived from the repetition of selected amino acid domains of extracellular mat ...

Regenerative medicine is an emerging field aiming to the development of new reparative strategies to treat degenerative diseases, injury, and trauma through developmental pathways in order to rebuild the architecture of the original injured organ and take over its functionality. M ...

Advances in regenerative nanomedicine raise a host of ethical, legal, and social questions that healthcare providers and scientists will need to consider. These questions and concerns include definitions, appropriate applications, dual use, potential risks, regulations, and a ...

The molecular dynamics method is a powerful computer simulation technique which provides access to the detailed time evolution (trajectory) of a system in specified conditions, such as a particular temperature or pressure. The full trajectory of the system can be analyzed using statis ...

Cells respond to biochemical and mechanical stimuli through a series of steps that begin at the molecular, nanometre level, and translate finally in global cell response. Defects in biochemical- and/or mechanical-sensing, transduction or cellular response are the cause of multiple d ...

Quantum Dots (QDs) are a new class of semiconductor nanoparticulate luminophores, which are actively researched for novel applications in biology and nanomedicine. In this review, the recent progress in the design and applications of QD labels for in vitro and in vivo imaging of cells is prese ...

Tissue engineering creates biological tissues that aim to improve the function of diseased or damaged tissues. In this chapter, we examine the promise and shortcomings of “top-down” and “bottom-up” approaches for creating engineered biological tissues. In top-down approaches, the c ...

This chapter presents as a proof of concept the development of a nanosensor based on the localized surface plasmon resonance for the analysis of biomolecules. The method presented take advantage of the plasmon generated in the surrounding of gold nanoparticles (i.e., 100 nm) for the specific i ...

While in the last years applications of carbon nanotubes in the field of biotechnology have been largely proposed, so far biomedical applications of boron nitride nanotubes (BNNTs) are still totally unexplored. BNNTs show very interesting physical properties that might be exploited ...

This chapter describes a versatile approach to immobilize proteins and other biomolecules on reactive self-assembled monolayers on gold as a means to study interactions (forces) between these biomolecules and nonbiological entities. Biomolecules are either immobilized on t ...

Determining the contact angle of a liquid on a solid surface is a simple method to assess the surface wettability. The most common method to measure the contact angle of a liquid consists of capturing the profile of a sessile drop of a few microliters on the surface using an optical system. Currently, this is a ...

Protein adsorption from (aqueous) solutions onto a (solid) surface is a common process that takes place at biological interfaces. This phenomenon, that spontaneously occurs, changes the properties of the surface and can induce structural modifications on proteins. Proteins in solu ...

Electrospinning is one of the most versatile and effective tools to produce nanostructured fibers in the biomedical science fields. The nanofibrous structure with diameters from tens to hundreds of nanometers largely mimics the native extracellular matrix (ECM) of many tissues. Th ...

The large number of cytokines and growth factors implicated in the regulation of liver regeneration has led to the possibility of using these molecules in therapy, namely, in the case of recombinant human hepatocyte growth factor (rhHGF). The importance and potential clinical usefulness ...