丁香实验_LOGO
登录
提问
我要登录
|免费注册
丁香实验推荐阅读
Genetic Subunit Vaccines: A Novel Approach for Genetic Immunization

Induction of epitope-specific cytotoxic T lymphocyte (CTL) responses can improve the outcome of disease in a variety of model systems. However, natural immunity or that arising by immunization with whole proteins or organisms with multiple potential epitopes, may not always result in t ...

丁香实验推荐阅读
Microarray Databases: Storage and Retrieval of Microarray Data

Microarray experiments rely on and generate (1–3) quantities of data that are simply too large to be stored on a researcher’s desktop computer in spreadsheet format. A single microarray may have 40,000 spots. Associated with each spot may be as many as 40 different metrics, and an experimental seri ...

丁香实验推荐阅读
Fabrication of cDNA Microarrays

DNA microarray technology has been used successfully to detect the expression of many thousands of genes, to detect DNA polymorphisms, and to map genomic DNA clones (1–4). It permits quantitative analysis of RNAs transcribed from both known and unknown genes and allows one to compare gene expr ...

丁香实验推荐阅读
Transfection of the Human Malaria Parasite Plasmodium falciparum

Methods to transiently and stably transfect blood stages of the human malaria parasite Plasmodium falciparum have been developed and adapted for gene-knockout, allelic replacement, and transgene expression in this organism. These methods are detailed in this chapter, as are appro ...

丁香实验推荐阅读
Crossmatching by Complement-Dependent Lymphocytotoxicity

The presence of preformed donor-specific HLA antibodies detected by Complement-dependent cyto�toxicity (CDC) crossmatch assay is associated with a high incidence of hyperacute or accelerated rejection and remains one of the gold standard tests pre-transplant. The standard CDC ...

丁香实验推荐阅读
Screening for Antibodies Against MICA by Luminex Flow Cytometry

Antibodies against MICA have been found in organ transplant recipients and were found to be associated with decreased survival of kidney allografts. The MICA antibody screening assay is a Luminex-based solid phase immunoassay designed to detect IgG antibodies binding to beads pre-co ...

丁香实验推荐阅读
HLA Antibody Detection and Characterization by Solid Phase Immunoassays: Methods and Pitfalls

Solid phase immunoassays for the detection and characterization of HLA-specific antibodies provide greatly increased sensitivity, specificity, and time and reagent efficiency, compared to the traditionally used cell-based methods. Testing is performed using commercia ...

丁香实验推荐阅读
Detection of Allo-HLA Cross-Reactivity by Virus-specific Memory T-Cell Clones Using Single HLA-Transfected K562 Cells

The ability to directly measure virus-specific lymphocytes using fluorochrome-labeled tetrameric complexes has proven a great advancement for the transplantation field. Viral peptide/HLA tetrameric complexes allow the rapid generation of virus-specific clones using ...

丁香实验推荐阅读
Detection and Characterisation of Alloreactive T Cells

T cell alloreactivity is responsible for much of the morbidity and mortality associated with tissue transplantation and graft versus host disease. Immunoassays for ex vivo monitoring and quantitation of alloreactive T cells are being increasingly utilised to provide valuable in ...

丁香实验推荐阅读
The Major Histocompatibility Complex: A Paradigm for Studies of the Human Genome

The major histocompatibility complex (MHC) on chromosome 6 is one of the most intensively studied regions of the human genome and has many features which make it unique. It is the source of much research interest because of its role in autoimmune and infectious disease susceptibility, and of diag ...

丁香实验推荐阅读
Separation and Cryopreservation of Lymphocytes from Spleen and Lymph Node

Spleen and lymph node retrieved post-mortem from deceased organ donors are a rich source of lymphocytes. Storage of lymphocytes separated from these sources can be valuable where post-transplant testing (crossmatching) is required. DNA extraction from stored lymphocytes also al ...

丁香实验推荐阅读
Overview of the Killer Cell Immunoglobulin-Like Receptor System

Natural killer (NK) cells are more than simple killers and have been implicated in control and clearance of malignant and virally infected cells, regulation of adaptive immune responses, rejection of bone marrow transplants, and autoimmunity and the maintenance of pregnancy. Human NK c ...

丁香实验推荐阅读
The Lymphocyte Crossmatch by Flow Cytometry for Kidney Transplantation

The flow cytometric lymphocyte crossmatch is a standard technique for evaluating the compatibility of potential kidney transplant recipients and donors. Recipient serum is incubated with donor lymphocytes and the latter are analysed in a flow cytometer for the presence of bound IgG a ...

丁香实验推荐阅读
KIR Typing by Non-Sequencing Methods: Polymerase-Chain Reaction with Sequence-Specific Primers

The killer-cell immunoglobulin-like receptors (KIR), which enable NK cells to detect allogeneic target cells and abnormalities in the expression of self-HLA molecules, are encoded by genes that display extensive copy number variation. These variations in the KIR genotype are relev ...

丁香实验推荐阅读
Impact of HLA Matching and HLA Antibodies in Organ Transplantation: A Collaborative Transplant Study View

The Collaborative Transplant Study (CTS) was initiated in 1982 in Heidelberg, Germany, and originated from the need to gain further insight into the complex problems and risks involved in human organ transplantation. Currently, more than 400 transplant centers in 45 countries are contr ...

丁香实验推荐阅读
Analytical Methods for Disease Association Studies with Immunogenetic Data

Disease association studies involving highly polymorphic immunogenetic data may involve analyses at one or many units of analysis, including amino acid, allele, genotype and haplotype levels, as well as consideration of gene–gene or gene–environment interactions. The selecti ...

丁香实验推荐阅读
Analytical Methods for Immunogenetic Population Data

In this chapter, we describe analyses commonly applied to immunogenetic population data, along with software tools that are currently available to perform those analyses. Where possible, we focus on tools that have been developed specifically for the analysis of highly polymorphic im ...

丁香实验推荐阅读
Standard Methods for the Management of Immunogenetic Data

In this chapter, we outline some basic principles for the consistent management of immunogenetic data. These include the preparation of a single master data file that can serve as the basis for all subsequent analyses, a focus on the quality and homogeneity of the data to be analyzed, the documentat ...

丁香实验推荐阅读
High Resolution MICA Genotyping by Sequence-Based Typing (SBT)

We have developed a MICA typing method based on polymerase chain reaction (PCR) sequence-based typing and a computer program that determines the polymorphisms and distinguishes the GCT repeats in exon 5. One PCR amplification was performed to obtain templates of 2.2 kb, including exons 2, 3, 4, and 5 ...

丁香实验推荐阅读
Genotyping of Single Nucleotide Polymorphisms by 5 Nuclease Allelic Discrimination

Real-time quantitative PCR is an efficient method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). In this chapter, we describe the 5′ nuclease allelic discrimination assay for genotyping biallelic SNPs.

提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序