遗传学实验技术

At the current rate of technological progress, high-throughput sequencing of nucleic acids has become a commodity. These techniques are perfectly suitable for viral small RNAs sequencing and contribute to the understanding of many aspects of virus biology in the context of host–patho ...

RNA interference (RNAi) plays novel roles in both host antiviral defense and viral replication. It has been shown that some viruses can exploit the RNAi machinery for their own benefit by encoding for their own viral small RNAs. Here we present a collection of methods to study adenoviral small RNAs, s ...

High-throughput sequencing emerged as a powerful approach to characterize siRNA populations �generated by hosts in response to viral infections. Here we described an informatic pipeline visitor to analyze in-house large sequencing datasets generated from Illumina sequenc ...

microRNAs (miRNAs) of host and viral origin have been suggested to play important roles in the viral infectious cycle. The discovery of new viral miRNAs, and understanding how viral infection alters host miRNAs, has been greatly aided by Northern blot analysis. The Northern blot method is used to ...

While cloning and/or massive parallel sequencing of small RNAs represent powerful tools for the discovery of novel miRNAs, computational miRNA prediction represents a valuable alternative which can be performed with comparably little technical effort. This is especially true f ...

Double-stranded RNA (dsRNA) is the trigger of RNA interference (RNAi)-mediated gene regulation. Dicer processes dsRNAs into short interfering RNAs (siRNAs), which are incorporated into the effector RNA-induced silencing complex (RISC) and direct degradation of homologous ta ...

The RNA interference (RNAi) pathway plays an important role in antiviral immunity in insects. To �counteract the RNAi-mediated immune response of their hosts, several insect viruses, such as Flock house virus, Drosophila C virus, and Cricket paralysis virus, encode potent viral suppres ...

The host–virus interaction is a continuous coevolutionary race involving both host defence strategies and virus escape mechanisms. RNA silencing is one of the main processes employed by eukaryotic organisms to fight viruses. However, viruses encode suppressor proteins to counte ...

Reconstitution of RNA-inducing silencing complex (RISC) in vitro is a powerful biochemical technique to analyze crucial steps in RNA interference (RNAi) pathways. RISC contains an RNase enzyme, Argonaute, which is guided by small interfering RNA (siRNA) to recognize and silence its ta ...

RNA interference (RNAi) is a conserved sequence-specific gene silencing mechanism that is induced by double-stranded RNA (dsRNA). The development of methods that allow internalization of dsRNA and concomitant silencing of the desired gene has not stopped since the first demonstra ...

RNA interference (RNAi) is an evolutionarily conserved mechanism for sequence-specific target RNA degradation in animals and plants, which plays an essential role in gene regulation. In addition, it is believed to function as a defense against viruses and transposons. In recent years, R ...

RNA interference (RNAi)-based gene therapy for the treatment of HIV-1 infection provides a novel antiviral approach. For delivery of RNAi inducers to CD4+ T cells or CD34+ blood stem cells, lentiviral vectors are attractive because of their ability to transduce nondividing cells. In additi ...

Intranasal administration of synthetic siRNA is an effective modality of RNAi delivery for the prevention and therapy of respiratory diseases, including pulmonary infections. Vehicles used for nasal siRNA delivery include established as well as novel reagents, many of which have b ...

For effective RNA interference (RNAi)-based therapies against viral infection, particularly highly mutational viruses like HCV and HIV, combinational strategies that target multiple regions within a viral genome are required to prevent resistance. The use of lentiviral vect ...

RNA interference (RNAi) is a potent and specific gene silencing mechanism that utilizes small �double-stranded RNA intermediates (small interfering RNAs or siRNAs) to target homologous mRNA sequences for degradation. The therapeutic potential of RNAi for HIV infection has been de ...

The highly specific mechanism of RNA (RNAi) that inhibits the expression of disease genes is increasingly being harnessed to develop a new class of therapeutics for a wide variety of human maladies. The successful use of small interfering RNAs (siRNAs) for therapeutic purposes requires sa ...

Since viral pathogens represent a significant threat to human health, a better understanding of the cellular factors that impact infection would facilitate the development of therapeutics. The recent advent of RNA interference (RNAi) technology coupled with the ease and efficien ...

It is clear that viral entry, replication, and spread is a complex process involving a dialog between the virus and the targeted host cell. Viruses have evolved highly specific strategies to hijack cellular factors to promote their internalization, initiate their replication, and facil ...

Influenza infections are considered a global threat to public health and cause seasonal epidemics and recurring pandemics. High mutation rates facilitate the generation of viral escape mutants rendering vaccines and drugs directed against virus-encoded targets ineffectiv ...

Maintenance of telomere length is a crucial event for cells to survive and proliferate. Telomere length dynamics is an equilibrium between incomplete DNA replication and telomere elongation by telomerase or alternative pathways. Telomere length measurements are usually perfo ...