生物信息学技术

Abstract Table of Contents Materials Figures Literature Cited Abstract CGRP receptor binding may be measured using homogenates of cell membranes or intact cells. Here a microcentrifugation?based assay is

Abstract Table of Contents Materials Figures Literature Cited Abstract Chromatin immunoprecipitation (ChIP) is a powerful and widely applied technique for detecting the association of individual proteins

Abstract Table of Contents Materials Figures Literature Cited Abstract Hydroxyl?radical protein footprinting is a straightforward and direct method to map protein sites involved in macromolecular interact

Abstract Table of Contents Materials Literature Cited Abstract This unit describes the combination of chromatin immunoprecipitation (ChIP) with microarray hybridization to determine the genome?wide occupancy profile of a DNA?associated pro

Abstract Table of Contents Figures Literature Cited Abstract Cytokines are key regulatory molecules that serve as critical mediators of cell?to?cell communication. They play a central role in immune function and are important molecular tar

Abstract Table of Contents Figures Literature Cited Abstract Digital microscopy combines the equipment of classic light microscopy with a computerized imaging system, i.e., a digital camera. This technique comprises image formation by opti

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit provides guidelines on how to use steady?state fluorescence spectroscopy for the quantification of protein?protein interact

Abstract Table of Contents Materials Figures Literature Cited Abstract A very large, ever?increasing repertoire of actin?binding proteins regulates the assembly dynamics and the spatial organization of ac

Abstract Table of Contents Materials Figures Literature Cited Abstract Sequential Chromatin Immunoprecipitation (SeqChIP) is a powerful technique for analyzing the simultaneous association of two differen

Abstract Table of Contents Materials Literature Cited Abstract Two vascular systems, cardiovascular and lymphatic, maintain appropriate interstitial and intravascular fluid volume in the body. Each is endowed with innate physiologic respon

Abstract Table of Contents Materials Figures Literature Cited Abstract This protocol describes amide proton exchange experiments that probe for changes in solvent accessibility at protein?protein interfac

Abstract Table of Contents Materials Figures Literature Cited Abstract In this unit, a method is described that allows construction of multiantennary oligonucleotide glycoconjugates on a solid support. A

Abstract Table of Contents Materials Figures Literature Cited Abstract 7?Substituted 7?deazapurine (pyrrolopyrimidine) 2??deoxyribonucleosides are synthesized by stereoselective nucleobase anion glycosylati

Abstract Table of Contents Materials Figures Literature Cited Abstract Telomeres are repetitive DNA sequences at the end of each chromosome that provide stability and prevent end?to?end chromosome fusions

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a convenient functional uptake assay for GABA transport into cell lines transiently transfected with GABA transpo

Abstract Table of Contents Materials Figures Literature Cited Abstract Semisynthetic DNA?streptavidin conjugates are synthesized by covalent coupling of thiol?modified DNA oligonucleotides and streptavidi

Abstract Table of Contents Materials Figures Literature Cited Abstract Sequence Tag Analysis of Genomic Enrichment (STAGE) is a method for experimentally identifying the in vivo chromosomal targets of DNA

Abstract Table of Contents Materials Literature Cited Abstract Many proteins bind to phospholipid membranes in order to gain or modify function. As an example, blood coagulation proteins must bind to phosphatidylserine?containing membranes

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes an extrinsic staining protocol using the amine?reactive CBQCA dye to measure the amount of protein on the surface

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a method to evaluate the effect that small molecules have on the binding interactions of a nuclear receptor prote