生物信息学技术

Abstract Table of Contents Materials Literature Cited Abstract The proteins of Saccharomyces cervsiae can be metabolically labeled, as described here, with 35methionine and 35cysteine or a hydrolysate of E. coli labeled with 35O42?. After th

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit provides protocols for prenylation and carboxy?methylation of proteins in cultured cells. These modifications often accompa

Abstract Table of Contents Materials Figures Literature Cited Abstract Covalent attachment of radiolabeled fatty acids (e.g., myristate or palmitate) is an alternative method for labeling proteins. This uni

Abstract Table of Contents Materials Literature Cited Abstract Post?translational modifications of proteins make it possible to determine where a protein normally resides or to follow its transport through the cell. One such modification i

Abstract Table of Contents Materials Literature Cited Abstract Metabolic labeling techniques are used to study the biosynthesis, processing, intracellular transport, secretion, degradation, and physical?chemical properties of proteins. Thi

Abstract Table of Contents Materials Figures Literature Cited Abstract Size?exclusion or gel filtration chromatography is one of the most popular methods for determining the sizes of proteins. Proteins in

Abstract Table of Contents Materials Figures Literature Cited Abstract The molecular weight of a protein is a basic characteristic that can only be approximated by techniques such as gel filtration and el

Abstract Table of Contents Materials Figures Literature Cited Abstract Arrays are used for parallel assays of large numbers of proteins in a single experiment. They provide an alternative to libraries for

Abstract Table of Contents Materials Figures Literature Cited Abstract Ligands can be introduced at the 5? terminus of an oligonucleotide by adding a linker to the ligand and modifying the 5? terminus of

Abstract Table of Contents Materials Figures Literature Cited Abstract Ligands can be introduced at the 5' terminus of an oligonucleotide by adding a linker to the ligand and modifying the 5' terminus of

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the use of size?exclusion chromatography with on?line light scattering, UV absorbance, and refractive index detec

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes far western blotting, a method of identifying protein?protein interactions. In a far western blot, one protein of

Abstract Table of Contents Materials Figures Literature Cited Abstract Voltage?dependent calcium channels are expressed in a variety of tissues including heart, muscles and brain. Saturation binding of a

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the procedures for measuring binding of a radiolabeled chemokine to chemokine receptors in cells or cell membrane

Abstract Table of Contents Materials Literature Cited Abstract The genetics of Drosophila is a powerful tool in the analysis of mutants and mutant proteins. Cultures of cells derived from wild?type or mutant flies can be pulse labeled to b

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit reports the synthesis of oligodeoxyribonucleotides covalently linked via their 5? termini to various ligands such as interc

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes methods for preparation of glycoproteins metabolically labeled with radioactive sugars, sulfate, and phosphate. M

Abstract Table of Contents Materials Figures Literature Cited Abstract Scintillation proximity assay (SPA) is a versatile homogeneous technique for radioactive assays which eliminates the need for separat

Abstract Table of Contents Materials Figures Literature Cited Abstract To successfully study chromatin structure and activity in vitro, it is essential to have a chromatin assembly system that will prepar

Abstract Table of Contents Materials Figures Literature Cited Abstract Reactions between protein molecules and reactive oxygen species (ROS) often lead to the modification of certain amino acid residues s