生物信息学技术

We present in this chapter the combined use of several recently introduced methodologies for the analysis of microarray datasets. These computational techniques are varied in type and very powerful when combined. We have selected a prostate cancer dataset which is available in the public ...

Human cytomegalovirus (HCMV) is one of the largest known DNA viruses. It is ubiquitous, and following resolution of primary productive infection, it persists in the human host by establishing a lifelong latent infection in myeloid lineage cells such as monocytes and their progenitors. Mo ...

Increasingly, the molecular genetics laboratory has to assess the biological significance of changes (variants) in a DNA sequence. Using the large genes BRCA1 and BRCA2 as examples, some approaches used to determine the biological significance of DNA variants are described. These inc ...

DNA sequencing is increasingly used in a range of medical activities involving DNA diagnostics and research. This is the result of improving technology and cheaper costs. Paradoxically, a greater demand for DNA sequencing has placed additional work on the laboratory because sequenci ...

Disease- and locus-specific variant databases have been a valuable resource to clinical and research geneticists. With the recent rapid developments in technologies, the number of DNA variants detected in a typical molecular genetics laboratory easily exceeds 1,000. To keep track of t ...

A problem frequently encountered by the biological scientist is the identification of a previously unknown gene or protein sequence, where there are few or no clues as to the biochemical function, ligand specificity, gene regulation, protein–protein interactions, tissue specific ...

Standardized methods for the solubilization of proteins prior to proteomics analyses incorporating two-dimensional gel electrophoresis (2-DE) are essential for providing reproducible data that can be subjected to rigorous statistical interrogation for comparative s ...

Peptide mass fingerprinting is a simple, quick, cheap, and relatively effective method of identifying proteins from mass spectrometry data. Proteins extracted from the complex mixture comprising the proteome of a sample are individually digested with a proteolytic enzyme into a ser ...

Searching online resources can provide medical researchers with an efficient means of gathering existing knowledge on the molecular causes of disease. The researcher may choose to explore the following areas, e.g., genetic mutations associated with the disease, function and cellul ...

In the post-Human Genome Project era, awareness of the resources available through the internet is essential to both molecular biologists and clinicians. An overview of the main databases and analytical tools described in this chapter is important to understand the principles upon whi ...

There is a growing demand for tools to support clinicians utilize genomic results generated by molecular diagnostic and cytogenetic methods in support of their decision-making. This chapter reviews existing experience and methods for the design, implementation and evaluation of ...

Complex diseases can involve the interaction of multiple genes and environmental factors. Discovering these genes is difficult, and in silico based strategies can significantly improve their detection. Data mining and automated tracking of new knowledge facilitate locus mapp ...

eConsulting, in all its contexts, can promote and improve the amount and quality of services and knowledge transferred to and among the community of health care providers and consumers. It can also improve the efficiency and effectiveness of the specialist and generalist workforce and acc ...

摘要：这是王泛森院士写的一篇文章，我觉得对即将读研的同学很有意义，到了研究生阶段，不能再用本科生的思维方式去学习，更应该具备学术研究素质，学会创新，学会主动学习，学会怎样与老师一起进入研究领域，又是人生中的一个新课题。一、研究生与大学生的区别首先跟大家说明一下研究生和大学生的区别。大学生基本上是来接受学问、接受知识的，然而不管是对于硕士时期或是博士时期的研究而言，都应该准备要开始制造新的知识，我们在美国得到博士学 ...

本文对 2006～2012 年国家自然科学基金委员会生命科学部及医学部面上项目资助情况进行整体分析，包括自然科学基金委对生命科学部及医学部的总投入经费情况，生命科学部及医学部面上项目每年增长情况，在此基础上分析了生命科学部及医学部各学科获资助动态，以期为生命科学及医学方面的科研项目申请者了解生命科学部及医学部的面上项目整体资助态势，并把握各学科领域（或资助方向）申请项目的难易程度提供参考。1 国家自然科学基金委员会对 ...

SPRi实时检测淋巴细胞和抗体芯片的相互作用Emmanuel SURANITI1, Elodie SOLLIER2, Roberto CALEMCZUK1, Thierry LIVACHE1, Patrice MARCHE2, Marie-Bernadette VILLIERS2, Yoann ROUPIOZ11 CREAB,UMR 5819 (CEA-CNRS-UJF), DRFMC, CEA Grenoble France.2 INSERM U823,equipe 8, Institut Albert Bonn ...

近年，关于门诊就诊流程的优化一直是各大中型医院努力解决的问题。门诊排队系统、门诊预付款（一卡通）系统、自助挂号系统、自动包药机等信息系统的应用在很大程度上缩短了病人等候时间，改善了就诊环境和秩序，提高了病人满意度，但这些系统对于提高门诊医生的工作效率却作用甚微。在门诊量日益增大的医院，每位门诊医生每天要接诊大量的病人，门诊诊疗效率的提高对于减轻医生工作强度、缩短病人等候时间具有重要意义。易迅电子病历--门诊电子病 ...

如何查找特定基因序列的心得与大家共享，下面以小鼠EDNRB基因为例子说明：1. 查找一个基因的启动子之前，最好是要对这个基因做一个初步的了解，了解方法为：http://www.ncbi.nlm.nih.gov/sites/entrez/上面查找ednrb注意：在research下面选择 Gene 在for后面输入ednrb2. 这个是查找结果，由于很多哺乳类动物都有该基因，查找结果显示了很多五种的ednrb基因，我们选择其中的Mus m ...

DNAMAN 是美国 Lynnon Biosoft 公司开发的高度集成化的分子生物学综合应用软件，可以用于多序列比对、PCR 引物设计、限制性酶切分析、质粒绘图、蛋白质分析等，几乎囊括了所有日常核酸、蛋白质序列的分析工作。因此掌握它，是从事分子生物学工作的必备。本文系丁香园站友gxumxc整理，若有不当之处，敬请指出。图1图2图3图4图5图6图7图8图9图10图11原帖链接：http://www.dxy.cn/bbs/topic/1205 ...

Abstract Table of Contents Figures Literature Cited Abstract In this article we describe the main characteristics and peculiarities of the different strains and stocks of laboratory animals from the genetic point of view. We explain how th