名称：大鼠灌注取脑 标准操作规程(SOP)关键词：大鼠 灌注取脑目的： 完整取出大鼠脑部（取材）操作步骤流程：1)麻醉 2)开胸 3)心脏左心室穿针剪开右心耳 4)生理盐水冲水 5)4%多基甲醛固定 6)取脑 7)保存或切片.1 多聚甲醛的配置：一般方法为：4％多聚甲醛PBS缓冲液配法：称取40g PFA溶于装有500ml DEPC水的玻璃容器（烧杯或烧瓶）中，持续加热磁力搅拌至60～65℃，使成 ...
名称：豚鼠饲育管理标准操作规程(SOP)关键词：豚鼠饲育管理目的：本操作规程是关于怎样饲育豚鼠才能使其被最有效的用于实验的具体操作规程。主体内容：1．饲育管理指标(1)环境卫生管理的指标(2)外观健康检查指标(3) 生产数量指标 按平面饲养设备，每人负责饲养管理种母为150只，种公30只，育种60只(其中母种50只，公种10只)：，月上交任务120只(月单位生产指标o．8只)。(4)日粮指标 成令 ...
1、 选取7~8周龄雌性小鼠，阴道口封闭，作为供体，下午3:00左右，每只小鼠腹腔注射PMSG（10 IU）。2、 47~48小时后，每只小鼠腹腔注射HCG（0.8 IU），并与正常公鼠合笼；另取数只适龄母鼠（2月龄以上）作为受体，阴道口潮红，与结扎公鼠合笼。3、第二天上午9:00前观察供体、受体，有精栓者拿出备用。受体笼拿出作好隔离措施。4、10:30左右，断颈处死供体，手术取出整个输卵管，放入 ...
麻醉（anesthesia）的基本任务是消除实验过程中所至的疼痛和不适感觉，保障实验动物的安全，使动物在实验中服从操作，确保实验顺利进行。一、常用的麻醉药（一）常用局部麻醉剂：普鲁卡因，此药毒性小，见效快，常用于局部浸润麻醉，用时配成0.5％～1％；利多卡因，此药见效快，组织穿透性好，常用1％～2％溶液作为大动物神经干阻滞麻醉，也可用0.25％～0.5％溶液作局部浸润麻醉。（二）常用全身麻醉剂：1 ...
名称：转基因小鼠模型的建立(SOP)关键词：转基因小鼠目的：在动物体研究转化基因原理：转基因动物是指染色体基因组中整合有外源基因并能遗传给后代的一类动物。整合到动物染色体基因组的外源基因称为转基因。转基因技术则是指制备转基因动物所需的一套技术，它涉及外源基因的构建、载体和受体的筛选、基因导人技术、供转基因胚胎发育的体外培养系统和宿主动物等许多方面。当制备转基因动物时，外源基因可能只整合到动物的部分 ...
Geraldine Seydoux and Andrew Fire Adapted from Seydoux G. and Fire A. (1995). Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos. In C. elegans: Modern Biological Analysi ...
MaterialsCaenorhabditis eleganscultures on agar platesE. colistrain OP50NG agar platesC. elegansRinger's solutionMicroscope (preferrably Nomarski interference or phase)Slides and coverslipsProcedur ...
Grow yeast overnight. Spin down 1.5 ml of culture in microfuge tubes. Decant. Add 250 µl of Qiagen buffer P1 and about 250 µl of glass beads to each tube. Beat or vortex on high for 3-5 minutes. Add 2 ...
1-2 g fresh material freezer-dried ground with 0.2g sand (if necessary) and then homogenized with 10ml RNA extraction buffer (see below). Spin at 8000rpm 4oC for 10 minutes Remove the supernatants to ...
1. Grind 20-50 frozen flies with a mortar and pestle in N2(l) and suspend in 2 ml Lysis Buffer. 2.Add Proteinase K to 100 mg/ml; incubate 1-2h @ 37oC with occassional swirling. 3. Phenol extract GENTL ...
Animals are normally euthanized at the end of a study for the purpose of sample collection or post-mortem examination. Animals may be euthanized because they are experiencing pain or distress. Euth ...
General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa. Experimental surgery has great potential for causing pain or distress to an ...
RationaleThe use of mouse genetic models requires an efficient system of unique animal identifiers. The means that tissue must be obtained from each animal for genotyping and that each animal must be ...
显微注射用BAC DNA的制备关于赛业（广州）生物科技 赛业（广州）生物科技有限公司 Cyagen Biosciences(Guangzhou) Inc.是美国 Cyagen Biosciences Inc. 旗下中国区子公司，专业从事干细胞、分子生物学及转基因模式动物的前沿技术研发。是华南地区唯一一家专门从事干细胞和转基因小鼠产业化的高新技术企业，在科研、管理、场地、设备、材料、及市场 ...
显微注射用质粒DNA的制备关于赛业（广州）生物科技 赛业（广州）生物科技有限公司 Cyagen Biosciences(Guangzhou) Inc.是美国 Cyagen Biosciences Inc. 旗下中国区子公司，专业从事干细胞、分子生物学及转基因模式动物的前沿技术研发。是华南地区唯一一家专门从事干细胞和转基因小鼠产业化的高新技术企业，在科研、管理、场地、设备、材料、及市场化工作 ...
day 1 1. Start 75 mL overnight cultures of agro (strain GV3101 C58C1 Rifr pMP90 Gmr Koncz & Schell) in YEP in 250 mL baffle flasks. 2. Grow at 28 °C shaking. 3. Prepare: ~ 3.5 L sterile H2O chilled to ...
This protocol is adapted from protocols by Nicole Bechtold (Bechtold et al. 1993) Andrew Bent (Bent et al. 1994) and Takashi Araki. No claims are made that any of the steps are necessary or ideal; th ...
1. Immerse Arabidopsis seeds in 10% Household Bleach for 20 min. 2. Rinse the seeds twice with 500 ml of sterile ddH2O and allow them to dry overnight in a laminar flow hood. 3. Push the resulting cru ...
Using this procedure it is possible to follow the development of pollen mother cells through to mature pollen. Sterile males were made using irradiation mutagenesis of Landsberg erecta seed. Buds harv ...
Materials: fixative dependent on antigen (common: 4% formaldehyde or 100% MeOH) post-fixative dependent on antigen (usually MeOH) PBS 1X PBS PBST PBS + detergent (0.05 to 0.5 % Tween or Triton) DAPI ( ...
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