免疫学技术

This protocol describes our method for preparing cells for immunofluorescence in which all incubations and washes are performed in microtiter dishes. Two dishes can be processed simultaneously. The la ...

DescriptionThis is the method for direct immunofluorescence labeling; that is the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling. Procedure ...

Direct Immunofluorescence Staining cells with antibodies directly linked to fluorochromes is known as direct immunofluorescence (DIF). DIF lends itself to multicolour experiments where a cell suspensi ...

This protocol is recommended for rat tissue fixed by paraformaldehyde-picric acid perfusion Tissue preparation:Subjects transcardially perfused with heparanized saline (0.9 w/v NaCl) followed by 4 w/v ...

BackgroundThis is the method for indirect immunofluorescence labeling; that is the antibodies do not have the fluorescent dye attached. Indirect labeling is more involved than direct labeling. If you ...

Supplies:Ethanol 100 USP (highest quality)FACS Staining Buffer (1XPBS w/ 3 calf serum 0.05 azide--filtered) ―Dilute staining antibodies in BufferDNAse (Sigma D-5025 Bovine Pancreas)RNase (Boehringer 2 ...

Chemicals and equipmentsActin Buffer ( 20 mM KPO4 10 mM PIPES 5 mM EGTA 2 mM MgCl2 pH 6.8) Assay buffer (Actin Buffer with 3.7 formaldehyde 0.1 triton 200X TRITC- Phaloidin from 50 mM stock.) DB Prep ...

The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample whether it is from the serum of an immunized animal or the cell supernatant from growing h ...

The sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites capable of binding to antibody since at least ...

OUTLINEClodronate (dichloromethylene diphosphonate) is used for treatment of osteolytic diseases and osteoporosis Its effect is due to inhibittion of the function of osteoclasts. This drug is also tak ...

OUTLINEClodronate (dichloromethylene diphosphonate) is used for treatment of osteolytic diseases and osteoporosis Its effect is due to inhibittion of the function of osteoclasts. This drug is also tak ...

MATERIALS10ml sterile syringes (one for each mouse) 20 gauge needles (one for each mouse) Phosphate Buffered Saline pH 7.2: PBS 1X (GibcoBRL Cat. No. 20012-027) Isoflurane for anesthetizing 70 Ethano ...

This protocol is used in our lab to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protocol contains 40-70 monocytes. This cell suspension is than use ...

OUTLINEThis modification of peritoneal macrophage preparation is designed for a subsequent thrombophagocytosis test. PROTOCOLSacrifice a mouse (use dry ice CO2) Sink a mouse in the 5 water solution o ...

Cut sections at 4µm use a clean water bath with distilled water and let the sections dry upright in order to facilitate adhesion between the section and the charged glass surface. (Slides: Fisher Supe ...

Cut sections at 4µm use a clean water bath with distilled water and let the sections dry upright in order to facilitate adhesion between the section and the charged glass surface. (Slides: Fisher Supe ...

Source of unwanted staining besides poor knowledge of the antibody reactivity and malice is due to: Endogenous enzymes or fluorochromes. Endogenous biotin. Endogenous antibody binding activity (Fc rec ...

Introduction: This method for the detection of cellular proliferation includes several modifications of a previously published protocol (Hayashi et al. 1988 J. Histochem. Cytochem. 36:511-514). This m ...

VisionTo provide scientific collaboration of excellence to National Toxicology Program (NTP) (http://ntp.niehs.nih.gov/) interdisciplinary research programs and DIR research using new technologies in ...

Rationale: The use of DNAse to improve nuclear antigen staining has been published long before the AR era 1 2.DNAse treatment is currently suggested as an unmasking technique for incorporated BrdU nuc ...