免疫学技术

网址:www.westang.com 电话：021-65333639 55229872豚鼠细胞因子分装ELISA试剂盒F26100豚鼠内皮素-1(Guinea pig ET-1)ELISA48T/96T2000/3000进口分装F26110豚鼠表皮生长因子(Guinea pig EGF)ELISA48T/96T1800/2800进口分装F26120豚鼠促卵泡素(Guinea pig FSH)EL ...

网址:www.westang.com 电话：021-65333639 55229872狗细胞因子分装ELISA试剂盒F28100狗脂联素(Canine adiponectin)ELISA48T/96T2000/3000进口分装F28110狗C-反应蛋白(Canine CRP)ELISA48T/96T1800/2800进口分装F28120狗表皮生长因子(Canine EGF)ELISA48T/9 ...

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选择3~5轮后，随机挑取克隆加入96孔微量滴定板中 并诱导表达可溶性scFv.然后，在包被有相关抗原的96孔微量滴定板中，用ELISA分析含有可溶性scFv的细菌上清。此过程可以识别出那些结合了抗原的scFv.但即便采用上述严谨性选择后，ELISA阳性克隆中仅有部分克隆的亲和力比野生型高。ELISA信号强度不能很好地显示哪个克隆具有较低的Ka值，而且更为普 遍的是它与scFv表达水平相关。因此， ...

● PCR试剂和设备● cFv基因文库单链模板DNA制备自pHENl中的天然scFv文库（10ng/u1）● Gelleclean试剂盒（Qbiogene）● Wtzard PCR纯化试剂盒（ProlneSa）● RJHl/2Xho引物： 5'-GGC ACC CTG GTC ACC GTC TCG AGT GGT GGA-3'● RJH3Xho引物： 5'-GGG ACA ATG ...

● PCR试剂和设备● 含有起始scFV基因的载体pHENl单链模板DNA（50ng/ul）● Geneclean试剂盒● Wlzard PCR纯化试剂盒● scFvJHL2XhoFOR引物：5'-GAG TCA TTC TCG TCT CGA GAC GGT GAC CAG GGT GCC-3'● scFvJH3XhoFOR引物： 5-GAG TCA TTC TCG TCT CGA ...

Purpose and backgroundsAbout nuclide...35-SDecay: (b-ray 0.167 MeV)Half life: 87.5 daysThick acrylic shield can block most of the emission. Because of the low energy it is impossible todetect spillage ...

Purpose...Concentration of proteins in a crude preparations (such as culture sup) can be estimated semi-quantitatively by using "Dot Blot" method if you have both purified protein and specific antibod ...

PurposeMaterialsAntibody 7E3 2L sup grown in flasks frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00) 50 mM Tris pH7.8 40 g ammonium sulfate for every 10 ...

PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid quantitative multiparameter analyses on singl ...

PurposeThis is a guideline for animal experiment. ProcedureAnimal Protocol An active animal protocol is needed for any experiment involving animals. If the experiment is not covered by the existing an ...

IntroductionGreen fluorescent proteins (GFP) and derivates thereof are widely used to study protein localization and dynamics in living cells (Heim and Tsien 1996; Tsien 1998). The validation and inte ...

IntroductionRNA-protein interactions play important roles within the cell. Using a variation of the widely-used chromatin immunoprecipitation (ChIP) assay the potential association of cellular RNAs an ...

Sections of paraformaldehyde fixed OCT-embedded vascular tissue are sectioned at 7 to 10 mm and stored desiccated at -70°C until needed. Thaw slides immediately before use usually 10-30 min. prior to ...

Sections of paraformaldehyde fixed OCT-embedded vascular tissue are sectioned at 7 to 10 mm and stored desiccated at -70°C until needed. Thaw slides immediately before use usually 10-30 min. prior to ...

Conjugation of carrier (BSA) to peptide1. Dissolve pure BSA @ 20mg/ml in 10mM phosphate pH 7.0.2. Dissolve MBS-SMCC @ 25mg/ml in NNdimethylformamide.3. Mix 20ml SMCC and 200ml BSA (500mg:4000mg or 1: ...

The ELISA protocols for detection of the antibody binding to an antigen-coated microtitre plate are standard laboratory techniques and will not be described here. We will just mention that most recomb ...

There are many different types of ELISAs which can detect the presence of protein in serum or supernatent. One of the most common types of ELISA is the so-called "sandwich ELISA." It is termed this be ...

You will need the following solutions: PBS (Phosphate-Buffered Saline Ca/Mg free) :- Solute1x10xNaCl8g80gKCl0.2g2gNa2PO4 (anhydrous)1.15g11.5gKH2PO4 (anhydrous)0.2g2gN.B.: Na2HPO4.2H2O can also be use ...