免疫学技术

Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies with the sensitivity of simple enzyme assays by using antibodies or antigens coupled to an easily-assayed enzyme. ELIS ...

Buffers and Reagents:Bicarbonate/carbonate coating buffer (100 mM) - antigen orantibodyshould be diluted in coating buffer toimmobilize them to the wells: 3.03 g Na2CO3 6.0 g NaHCO3 (1000 ml distill ...

1. Materials(1) TS Buffer: 10 mM Tris pH 8.0 150 mM NaCl(2) TST Buffer: TS Buffer with 0.05% Tween-20(3) Alkaline Phosphatase Buffer: 50 mM NaCarbonate 1 mM MgCl2 (Bring to pH 9.8 with 1 N HCl)(4) AP ...

Interleukin-2 (IL-2) is a multi-functional protein most notably known for the ability to induce the proliferation and maturation of activated T cells. Additional biologicalfunctions include:Stimulus f ...

Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC.Mat ...

时间分辨荧光免疫分析技术及临床应用.pdf

随着分析方法的飞速发展，无论是食品中有毒有害物质，还是环境中痕量元素的检测，或者生物体内功能因子的分析，都迫切需要一种灵敏度高、快速准确、性能稳定的痕量分析方法。时间分辨荧光免疫分析技术（time-resolved fluoroimmunoassay，简称为TRFIA）是20世纪80 年代中期发展起来的一种新的荧光标记技术。这种方法应用某些特殊的稀土金属，能够区分背景光的散射所引起的干扰，从而大大 ...

时间分辨荧光免疫分析（time resolved luoro-immunoassay，TrFIA）技术出现于20世纪80年代初期，是在免疫荧光分析的基础上发展的一种新型超微量物质免疫分析方法。 TrFIA采用镧系稀土元素标记抗体，利用荧光发射体的荧光寿命差别将波长相同的荧光分开，排除样品中的非特异荧光的干扰，从而最大限度地提高分析方法的灵敏度。该方法在灵敏度、特异性、稳定性及测量自动化等方面可 ...

氯霉素的高灵敏时间分辨荧光免疫分析.pdf

General comments: Antibodies like most proteins do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration ...

Materials6gm glycerol 2.4gm mowiol 6ml distilled water 12ml Tris buffer 0.2M at pH 8.5 Method Weigh out the glycerol in a 50ml plastic tube and add the mowiol. Stir thoroughly. Add water and leave for ...

Preface:You should not co-stain in IHC antigens located on the same structure (nucleus membrane cytoplasm). That because the success of this double staining procedure is based on the complete develo ...

Biological specimens are embedded in resin so that they can be thin sectioned. Many different resin formulas are currently available and all have been used for post-embedding labeling (i.e. where anti ...

Equipment and chemicalsZeiss inverted microscope eppendolf microinjector eppendolf micromanipulator (in Dr. Murphy’s lab)Eppendolf femtotips 100mM cAMP in PMDB buffer PM Buffer.Preparing Cellsa. Wash ...

以酶联免疫反应与碱性去磷酸酶呈色反应侦测杂合讯息 进行杂合反应时所使用的探针若为放射性物质所标定者，杂合讯息可经由放射显影术 (autoradiography) 侦测出來；若为DIG所标定者，则需借助anti-DIG抗体与碱性去磷酸酶 (alkaline phosphatase AP) 的conjugate，并利用碱性去磷酸酶的酵素活性转现杂合反应讯息。 目前常用來进行碱性去磷酸酶呈色 ...

人细胞因子分装ELISA试剂盒货号品名方法规格价格备注F00010血管紧张素转化酶(Human ACE)ELISA48T/96T2000/3000进口分装F00020激活素A(Activin A)ELISA48T/96T2000/3000进口分装F00021雄激素结合蛋白(ABP)ELISA48T/96T1800/2800进口分装F00022促肾上腺皮质激素(ACTH)ELISA48T/96T ...

小鼠细胞因子分装ELISA试剂盒　　　　货号品名方法规格价格备注F10000小鼠血管紧张素转化酶(mouse ACE)ELISA48T/96T2000/3000进口分装F10001小鼠雄激素结合蛋白(mouse ABP)ELISA48T/96T2000/3000进口分装F10002小鼠促肾上腺皮质激素(mouse ACTH)ELISA48T/96T1800/2800进口分装F10010小鼠Acti ...

大鼠细胞因子分装ELISA试剂盒　　　　货号品名方法规格价格备注F15000大鼠Active Caspase-3ELISA48T/96T2000/3000进口分装F15010大鼠Active Caspase-7ELISA48T/96T2000/3000进口分装F15020大鼠Active Caspase-8ELISA48T/96T2000/3000进口分装F15030大鼠激活素A(rat Acti ...

兔细胞因子分装ELISA试剂盒　　　　货号品名方法规格价格备注F19011兔子脂联素(rabbit adiponectin)ELISA48T/96T1800/2800进口分装F19021兔骨成型蛋白-2(rabbit BMP-2)ELISA48T/96T2000/3000进口分装F19031兔子脑钠素/脑钠尿肽(rabbit BNP)ELISA48T/96T2000/3000进口分装F19051兔 ...

猪细胞因子分装ELISA试剂盒　　　　货号品名方法规格价格备注F25000猪促肾上腺皮质激素(Swine ACTH)ELISA48T/96T2000/3000进口分装F25001猪脑钠素/脑钠尿肽(Swine BNP)ELISA48T/96T2000/3000进口分装F25002猪III型胶原(Swine Collagen Type III)ELISA48T/96T1800/2800进口分装F25 ...