蛋白质技术

Native Acrylamide Gels (35 ml)3.5%5%6%8% 30/0.8% acrylamide 4.1 ml 5.8 ml7 ml9.3 ml 10X TBE 3.5 ml3.5 ml3.5 ml 3.5 mlH20 27.4 ml25.7 ml24.5 ml22.2 mlDegas 1-2 minAdd 0.25 ml 10% ammonium persulfateAd ...

This is a method for the separation and identification of proteins in a sample by displacement in 2 dimensions oriented at right angles to one another. This allows the sample to separate over a larger ...

DefinitionAmino acids nucleotides polypeptides and other compounds in a colloidal state can be separated by the application of external voltages which cause charged colloid particles to move toward an ...

OverviewThis protocol is a detail description of the procedure in performing 2D gel electrophoresis for illustrating the protein profile of the whole chicken egg. A similar protocol was presented ...

Intracellular virus proteinsThe following method has been developed principally for the analysis of intracellular proteins from picornavirus infected HEp-2C cell monolayers. Modifications to the incub ...

The following protocol has been developed for preparing soluble bacterial proteins in a form suitable for analysis by 2D-PAGE. The procedure was principally developed for the analysis of proteins ex ...

OverviewThis protocol is a detail description of the laboratory procedure in performing 2D gel electrophoresis for illustrating the protein profile of the human plasma proteome.Material1.Perip ...

1. Excision of protein bands (spots) from polyacrylamide gelsRinse the gloves you use with water to avoid traces of dust in your sample. Rinse the gel with water. Excise spots with clean pipette tip ...

OverviewThis protocol is a detail description of the laboratory procedure in performing 2D gel electrophoresis for illustrating the protein profile of the human plasma proteome.Material1.Perip ...

For an in-depth review of the method see Friedman K. and B. Brewer (1995) Analysis of replication intermediates by two-dimensional agarose gel electrophoresis. Meth. Enzymol. 262: 613-627. Two dimensi ...

For an in-depth review of the method see Friedman K. and B. Brewer (1995) Analysis of replication intermediates by two-dimensional agarose gel electrophoresis. Meth. Enzymol. 262: 613-627. Two dimensi ...

ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall: 165 x 130 mmMedium: 165 x 200 mmLarge: 165 x 260 mm5% Analytical GelsReagent1 mm Small1 mm Medium1 mm Large1 ...

ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELSGel SizesSmall: 165 x 130 mmMedium: 165 x 200 mmLarge: 165 x 260 mm5% Analytical GelsReagent1 mm Small1 mm Medium1 mm Large1 ...

Preparation of denaturing 6% polyacrylamide gels for microsatellite analysis(also for SSAP high-resolution IRAP/ISSR and other analyses)Saadiah Jamli and Pat Heslop-Harrison November 2003University of ...

Preparation of denaturing 6% polyacrylamide gels for microsatellite analysis(also for SSAP high-resolution IRAP/ISSR and other analyses)Saadiah Jamli and Pat Heslop-Harrison November 2003University of ...

等电聚焦电泳与琼脂糖电泳过程中凝胶板的制备方法和简单操作等电聚焦电泳１.制备凝胶板1)30%凝胶母液的制备丙烯酰胺30克，N、N双甲叉丙烯酰胺0.8克，加蒸馏水至100毫升，溶解后过滤贮存于有色瓶内．2)准备制胶模具准备玻璃板凉快，相应压条三根．同样大小的玻璃纸及聚碳酸脂薄膜各一张（也可不用）．文具夹若干．上述用品洗净晾干后备用．先将玻璃纸用蒸馏水浸透，平放在一块玻璃上，放三根压条于其上（这样就形 ...

Weigh out the desired amount of agarose and place in an Erlenmeyer flask with a measured amount of electrophoresis buffer e.g. for an 0.8% gel add 0.8 gm of agarose and 100ml of TBE Buffer (1X) to a 2 ...

等电聚焦电泳与琼脂糖电泳过程中凝胶板的制备方法和简单操作等电聚焦电泳１.制备凝胶板1)30%凝胶母液的制备丙烯酰胺30克，N、N双甲叉丙烯酰胺0.8克，加蒸馏水至100毫升，溶解后过滤贮存于有色瓶内．2)准备制胶模具准备玻璃板凉快，相应压条三根．同样大小的玻璃纸及聚碳酸脂薄膜各一张（也可不用）．文具夹若干．上述用品洗净晾干后备用．先将玻璃纸用蒸馏水浸透，平放在一块玻璃上，放三根压条于其上（这样就形 ...

正文保留时间不重现有两种不同的情况：既保留时间漂移和保留时间波动。前者是指保留时间仅沿单方向发生变化，而后者指保留时间无固定规律的波动。将此两种情况区分开来对找到问题的原因往往很有帮助。如，保留时间的漂移往往由柱老化引起；而柱老化不可能引起保留时间的无规律波动。事实上，保留时间漂移的多半原因是不同机理的色谱柱老化，如固定相流失（例如通过水解），色谱柱污染（由样品或流动相所致）等。保留时间漂移的几种 ...

正文保留时间不重现有两种不同的情况：既保留时间漂移和保留时间波动。前者是指保留时间仅沿单方向发生变化，而后者指保留时间无固定规律的波动。将此两种情况区分开来对找到问题的原因往往很有帮助。如，保留时间的漂移往往由柱老化引起；而柱老化不可能引起保留时间的无规律波动。事实上，保留时间漂移的多半原因是不同机理的色谱柱老化，如固定相流失（例如通过水解），色谱柱污染（由样品或流动相所致）等。保留时间漂移的几种 ...