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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C, sealed storage, away from moisture
- 英文名:
Adenosine-5'-O-(3-thiotriphosphate) tetralithium salt; Adenosine 5'-[γ-thio]triphosphate tetralithium salt
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
93839-89-5
- 规格:
5 mg/10 mg/25 mg
| 规格: | 5 mg | 产品价格: | ¥997.0 |
|---|---|---|---|
| 规格: | 10 mg | 产品价格: | ¥1700.0 |
| 规格: | 25 mg | 产品价格: | ¥3750.0 |
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ATPγS tetralithium salt
CAS No. : 93839-89-5
MCE 国际站:ATPγS tetralithium salt
产品活性:ATPγS (tetralithium salt) 是真核生物翻译起始因子 eIF4A 的核苷酸水解和RNA解链活性的底物。
研究领域:Cell Cycle/DNA Damage
作用靶点:Eukaryotic Initiation Factor (eIF)
In Vitro: ATPγS (tetralithium salt) enhances intrinsic fluorescence and induces aggregation which increases the activity of spinach Rubisco activase.
?ATPγS (50-100 μM final blood concentration) attenuates inflammatory response with decreased accumulation of cells (48%, P < 0.01) and proteins (57%, P < 0.01) in bronchoalveolar lavage and reduces neutrophil infiltration and extravasation of Evans blue albumin dye into lung tissue.
In Vivo: ATPγS (tetralithium salt, 50 μM final, intravenous) demonstrates preserved lung parenchymal architecture.
?ATPγS results in a dose-dependent effect on EBA extravasation in LPS-treated mice.
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文献和实验ATP-γ-S (5 mM final). Reactions(4-6 µl) are analyzed by electrophoresis on a 5% non-denaturing PAGE buffered with 0.2X TBE at 4°C. Document the gel using an instrument capable of fluorescent imaging (note 3 ). Probing
(1.5 mM AlCl3 ) distilled water to 100 ml Store at room temperature 30 mM Mg GTP-g-S* 10 mg GTP-g-S tetralithium salt (available from Boehringer Mannheim #220 467) 18 ml 1 M MgSO4 Stock (30 mM MgSO4 ) add 572 ml distilled water (to 592 ml
General Preparation 1. Prepare buffers and have them cold. Make 50X stock of Buffer G and dilute as needed from frozen aliquots of 50X Buffer G. 1X Extraction Buffer without detergents: 1 M Tris-Cl, pH 7.0, 0.6 M KCl, 0.5 mM ATP
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