In Vitro: E67-2 (1~100 μM; 5 minutes) reduces inhibitory effect against GLP by a factor of approximately 1500, resulting in an IC50 of 75 µM. E67-2 has much reduced inhibition on PHF8 on the doubly methylated H3(1-24)K4me3K9me2 peptide substrate. E67-2 (100~100000 nM; 24 hours; fibroblasts) has significantly reduced cell toxicity .