反转录试剂盒 II

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反转录试剂盒 II

MCE 国际站：反转录试剂盒 II

RT Master Mix for qPCR II 采用升级款反转录酶，反应更高效、快速，适用于高 GC 含量、复杂二级结构、少量 RNA 模板等。即用型预混液，一步操作即可完成反转录反应，简便、快捷。

Description & Advantages:

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription. The 4× Super RT Mix contains all the reagents necessary for first-strand cDNA synthesis. With RNA added, the optimized system will provide sensitive and reliable cDNA synthesis. In addition, the increased heat resistance of the advanced reverse transcriptase allows reverse transcription to be performed at a higher temperature, increasing the efficiency and specificity of cDNA synthesis.

Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR and qPCR. MCE SYBR Green qPCR Master Mix (HY-K0501) is highly recommened for detection of the expression levels of interested genes.

 

Features:

1. Synthesize cDNA rapidly and specifically.

2. Only one transfer step reduces handling errors significantly.

3. Optimized buffer enhances sensitive and reliable cDNA synthesis.

4. Super-efficient reaction even with low template amounts (total RNA: 50 pg -5 μg in a 20 μL reaction).

5. The kit provides both Oligo dT Primer and Random Primer.