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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 保质期:
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- 英文名:
5-Bromo-2-pyridinemethanamine
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
173999-23-0
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验1/2 volume of 1-bromo-3-chloro-propane (molecular biology grade, SIGMA) as chloroform substitute. For efficient recovery of small RNAs, DNA LoBind tubes (Eppendorf) were used and all centrifugation steps were performed at maximum speed and 4°C in an Eppendorf 5417R
Recombineering‐Based Procedure for Creating BAC Transgene Constructs for Animals and Cell Lines
. The recombineering plasmid, pKD46, is used to fabricate BAC transgenic constructs that can be used in generating transgenic organisms as well as used in mammalian cell culture. Curr. Protoc. Mol. Biol. 95:23.14.1?23.14.28. © 2011 by John Wiley & Sons
ECD和戊二醛加入混合液,振摇后计时,用pH计检测,约2~3min时,pH降至6.6,再以1N的NaOH在4min内调pH至7.0,此时,将该混合固定液加入盛有细胞(经PBS漂洗过)的器皿中,在23℃固定7min后,以PBS洗去固定液,即可进一步处理。ECD即乙基-二甲基氨基丙基碳亚胺盐酸盐[1-ethyl-3(3-dimethyl-aminoprpyl)-carboi-imide hydrochloride],简称乙基-CDI,常用于多肽类激素的固定,对酶等蛋白质的固定也有良好效果。ECD单独应用











