18368-59-7;98%;3-Bromo-4-methy

【求助】蛋白激酶C的信号通路

9sMffkekXB4sNHvv7vvutpeWLrO//NLf2GOPL5F98YAbNRHkguTs7e23I6bPsgkHTbOZM2farKOOs23btslRRdkee2yJPfHkr23lyhX21f/2d3bHojvsTm3HHnu0Pf/i87ZixTIn8GulAb/yBz+0Y2bMsqt+8H2787bb7KQTZ9vqlSvtWRH6937g/Xb9gpusX2YhW3butKuvv86+8BeftZdfftlOPflke2HpUmnAV9nalWttZ1ePzEiY7eccWX+hKVB

MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

1/2 volume of 1-bromo-3-chloro-propane (molecular biology grade, SIGMA) as chloroform substitute. For efficient recovery of small RNAs, DNA LoBind tubes (Eppendorf) were used and all centrifugation steps were performed at maximum speed and 4°C in an Eppendorf 5417R

燕麦转基因及其在提高渗透胁迫耐受中的应用

磷酸酶缓冲液：100 mmol/L Tris-base，100 mmol/L NaCl，5 mmol/L  MgCl2( pH 9.8 ) 含有  0.33 mg/ml  NBT  (Nitro  blue  tetrazolium ) ，0.16 mg/ml BCIP (5 - bromo - 4 -chloro-3 -indolyl  phosphate)。 ( 7 ) GUS底物：10 mmol/L EDTA  ( pH 7.0 ) ，0.1 mol/L phosphate  buffer  (pH