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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
咨询
- 保质期:
咨询
- 英文名:
2-Bromo-3-chloronitrobenzene
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
19128-48-4
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
(Boehringer Mannheim Cat# 604 205, 812 854)- Triton X-100 (Sigma Cat# X-100) - X-phosphate: 5-Bromo-4-chloro-3-indolyl-phosphate. (Boehringer Mannheim Cat# 1383 221) B. Stock solutions DEPC treatment of solutions is not needed. Solutions are stored
磷酸酶缓冲液:100 mmol/L Tris-base,100 mmol/L NaCl,5 mmol/L MgCl2( pH 9.8 ) 含有 0.33 mg/ml NBT (Nitro blue tetrazolium ) ,0.16 mg/ml BCIP (5 - bromo - 4 -chloro-3 -indolyl phosphate)。 ( 7 ) GUS底物:10 mmol/L EDTA ( pH 7.0 ) ,0.1 mol/L phosphate buffer (pH
1/2 volume of 1-bromo-3-chloro-propane (molecular biology grade, SIGMA) as chloroform substitute. For efficient recovery of small RNAs, DNA LoBind tubes (Eppendorf) were used and all centrifugation steps were performed at maximum speed and 4°C in an Eppendorf 5417R






