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pENTR223-LTF人源基因质粒

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  • 2025年08月10日
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    • 英文名：

      BC015822

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      5年

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      上海烜雅生物科技有限公司

    • 保存条件：

      -20℃

    基本信息
    名称：pENTR223-LTF人源基因质粒
    别称BC015822
    复制子:pUC
    原核抗性:Spe
    克隆菌株:DH5a
    培养条件:37度 
    5'测序引物: M13F
    3'测序引物: M13R

     质粒属性
    载体宿主：大肠杆菌
    载体用途：PCR模板
    基因种属：人
    基因类型：ORF
    原核抗性：Spe
    真核抗性：
    荧光蛋白：

    质粒简介
       pDONR223-HDAC7（NM_001308090.1）是一个人源基因克隆质粒。

    质粒图谱
    产品细节图片1

    <span style="font-size:10.5pt"><span style="font-family:等线"><b><span style="font-family:&quot;微软雅黑&quot;,sans-serif">基本信息</span></b></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">名称：</span></span></span>pENTR223-HDAC7人源基因质粒<br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">别称</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">: </span></span></span></span>NM_001308090.1;HD7;HD7A;HDAC7A<br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">复制子</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">:</span></span></span></span>pUC<br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">原核抗性</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">:</span></span></span></span>Spe<br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">克隆菌株</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">:</span></span></span></span>DH5a<br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">培养条件</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">:</span></span></span></span>37度<br />
    <br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><b><span style="font-family:&quot;微软雅黑&quot;,sans-serif">质粒属性</span></b></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">载体宿主：</span></span></span>大肠杆菌<br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">载体用途：</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">基因种属：</span></span></span>人<br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">基因类型：</span></span></span>ORF<br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">原核抗性：</span></span></span>Spe<br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">真核抗性：</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">荧光蛋白：</span></span></span><br />
    <br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><b><span style="font-family:&quot;微软雅黑&quot;,sans-serif">质粒简介</span></b></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><b>&nbsp;&nbsp;&nbsp;</b></span></span>pDONR223-HDAC7（NM_001308090.1）是一个人源基因克隆质粒。<br />
    <br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><b><span style="font-family:&quot;微软雅黑&quot;,sans-serif">质粒图谱</span></b></span></span><br />
    <img alt="" src="https://img1.dxycdn.com/2021/0710/748/3018299019838851943-14.png" style="width: 400px; height: 419px;" /><br />
    <br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><b><span style="font-family:&quot;微软雅黑&quot;,sans-serif">质粒序列</span></b></span></span><br />
    LOCUS &nbsp; &nbsp; &nbsp; Exported &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;3620 bp ds-DNA &nbsp; &nbsp; circular SYN 05-DEC-2017<br />
    DEFINITION &nbsp;synthetic circular DNA<br />
    FEATURES &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Location/Qualifiers<br />
    &nbsp; &nbsp; &nbsp;source&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;1..3620<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/organism=&quot;synthetic DNA construct&quot;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/mol_type=&quot;otherDNA&quot;<br />
    &nbsp; &nbsp; &nbsp;terminator&nbsp; &nbsp; &nbsp;268..295<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/label=rrnB T2 terminator<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/note=&quot;transcription terminator T2 from the E. coli rrnB&nbsp;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;gene&quot;<br />
    &nbsp; &nbsp; &nbsp;terminator&nbsp; &nbsp; &nbsp;387..473<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/gene=&quot;Escherichia coli rrnB&quot;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/label=rrnB T1 terminator<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/note=&quot;transcription terminator T1 from the E. coli rrnB&nbsp;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;gene&quot;<br />
    &nbsp; &nbsp; &nbsp;protein_bind&nbsp; &nbsp;570..668<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/gene=&quot;mutant version of attL&quot;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/label=attL1<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/bound_moiety=&quot;LRClonase(TM)&quot;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/note=&quot;recombination site for the Gateway(R) LR reaction&quot;<br />
    &nbsp; &nbsp; &nbsp;misc_feature&nbsp; &nbsp;669..1498<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/label=HADC7<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/note=&quot;NM_001308090.1&quot;<br />
    &nbsp; &nbsp; &nbsp;protein_bind&nbsp; &nbsp;complement(1499..1597)<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/gene=&quot;mutant version of attL&quot;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/label=attL2<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/bound_moiety=&quot;LRClonase(TM)&quot;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/note=&quot;recombination site for the Gateway(R) LR reaction&quot;<br />
    &nbsp; &nbsp; &nbsp;CDS&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 2086..2877<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/codon_start=1<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/gene=&quot;aadA&quot;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/product=&quot;aminoglycosideadenylyltransferase (Murphy,&nbsp;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;1985)&quot;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/label=Spe<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/note=&quot;confers resistance to spectinomycin and&nbsp;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;streptomycin&quot;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/translation=&quot;MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK&quot;<br />
    &nbsp; &nbsp; &nbsp;rep_origin&nbsp; &nbsp; &nbsp;2970..3558<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/direction=RIGHT<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/label=ori<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;/note=&quot;high-copy-number ColE1/pMB1/pBR322/pUC origin of&nbsp;<br />
    &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;replication&quot;<br />
    ORIGIN<br />
    &nbsp; &nbsp; &nbsp; &nbsp; 1 ctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctga<br />
    &nbsp; &nbsp; &nbsp; &nbsp;61 taccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaga<br />
    &nbsp; &nbsp; &nbsp; 121 gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggca<br />
    &nbsp; &nbsp; &nbsp; 181 cgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatacgcgtaccgc<br />
    &nbsp; &nbsp; &nbsp; 241 tagccaggaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgctta<br />
    &nbsp; &nbsp; &nbsp; 301 gtttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttc<br />
    &nbsp; &nbsp; &nbsp; 361 acaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaa<br />
    &nbsp; &nbsp; &nbsp; 421 caacagataaaacgaaaggcccagtcttccgactgagcctttcgttttatttgatgcctg<br />
    &nbsp; &nbsp; &nbsp; 481 gcagttccctactctcgcgttaacgctagcatggatgttttcccagtcacgacgttgtaa<br />
    &nbsp; &nbsp; &nbsp; 541 aacgacggccagtcttaagctcgggcccccaaataatgattttattttgactgatagtga<br />
    &nbsp; &nbsp; &nbsp; 601 cctgttcgttgcaacaaattgatgagcaatgcttttttataatgccaactttgtacaaaa<br />
    &nbsp; &nbsp; &nbsp; 661 aagttggcatgggcttctgcttcttcaactcagtggccatcgcctgccggcagctgcaac<br />
    &nbsp; &nbsp; &nbsp; 721 agcagagcaaggccagcaagatcctcattgtagactgggacgtgcaccatggcaacggca<br />
    &nbsp; &nbsp; &nbsp; 781 cccagcaaaccttctaccaagaccccagtgtgctctacatctccctgcatcgccatgacg<br />
    &nbsp; &nbsp; &nbsp; 841 acggcaacttcttcccggggagtggggctgtggatgaggtaggggctggcagcggtgagg<br />
    &nbsp; &nbsp; &nbsp; 901 gcttcaatgtcaatgtggcctgggctggaggtctggacccccccatgggggatcctgagt<br />
    &nbsp; &nbsp; &nbsp; 961 acctggctgctttcaggatagtcgtgatgcccatcgcccgagagttctctccagacctag<br />
    &nbsp; &nbsp; &nbsp;1021 tcctggtgtctgctggatttgatgctgctgagggtcacccggccccactgggtggctacc<br />
    &nbsp; &nbsp; &nbsp;1081 atgtttctgccaaatgttttggatacatgacgcagcaactgatgaacctggcaggaggcg<br />
    &nbsp; &nbsp; &nbsp;1141 cagtggtgctggccttggagggtggccatgacctcacagccatctgtgacgcctctgagg<br />
    &nbsp; &nbsp; &nbsp;1201 cctgtgtggctgctcttctgggtaacagggtggatcccctttcagaagaaggctggaaac<br />
    &nbsp; &nbsp; &nbsp;1261 agaaacccaacctcaatgccatccgctctctggaggccgtgatccgggtgcacagtaaat<br />
    &nbsp; &nbsp; &nbsp;1321 actggggctgcatgcagcgcctggcctcctgtccagactcctgggtgcctagagtgccag<br />
    &nbsp; &nbsp; &nbsp;1381 gggctgacaaagaagaagtggaggcagtgaccgcactggcgtccctctctgtgggcatcc<br />
    &nbsp; &nbsp; &nbsp;1441 tggctgaagataggccctcggagcagctggtggaggaggaagaacctatgaatctctacc<br />
    &nbsp; &nbsp; &nbsp;1501 caactttcttgtacaaagttggcattataagaaagcattgcttatcaatttgttgcaacg<br />
    &nbsp; &nbsp; &nbsp;1561 aacaggtcactatcagtcaaaataaaatcattatttggccatccagctgatatcccctat<br />
    &nbsp; &nbsp; &nbsp;1621 agtgagtcgtattacatggtcatagctgtttcctggcagctctggcccgtgtctcaaaat<br />
    &nbsp; &nbsp; &nbsp;1681 ctctgatgttacattgcacaagataaaaatatatcatcatgcctcctctagaccagccag<br />
    &nbsp; &nbsp; &nbsp;1741 gacagaaatgcctcgacttcgctgctgcccaaggttgccgggtgacgcacaccgtggaaa<br />
    &nbsp; &nbsp; &nbsp;1801 cggatgaaggcacgaacccagtggacataagcctgttcggttcgtaagctgtaatgcaag<br />
    &nbsp; &nbsp; &nbsp;1861 tagcgtatgcgctcacgcaactggtccagaaccttgaccgaacgcagcggtggtaacggc<br />
    &nbsp; &nbsp; &nbsp;1921 gcagtggcggttttcatggcttgttatgactgtttttttggggtacagtctatgcctcgg<br />
    &nbsp; &nbsp; &nbsp;1981 gcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgatgttatggagcagcaac<br />
    &nbsp; &nbsp; &nbsp;2041 gatgttacgcagcagggcagtcgccctaaaacaaagttaaacatcatgagggaagcggtg<br />
    &nbsp; &nbsp; &nbsp;2101 atcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaa<br />
    &nbsp; &nbsp; &nbsp;2161 ccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacac<br />
    &nbsp; &nbsp; &nbsp;2221 agtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagct<br />
    &nbsp; &nbsp; &nbsp;2281 ttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgct<br />
    &nbsp; &nbsp; &nbsp;2341 gtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgc<br />
    &nbsp; &nbsp; &nbsp;2401 gaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagcc<br />
    &nbsp; &nbsp; &nbsp;2461 acgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttg<br />
    &nbsp; &nbsp; &nbsp;2521 gtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcg<br />
    &nbsp; &nbsp; &nbsp;2581 ctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaat<br />
    &nbsp; &nbsp; &nbsp;2641 gtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaag<br />
    &nbsp; &nbsp; &nbsp;2701 gatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatactt<br />
    &nbsp; &nbsp; &nbsp;2761 gaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcag<br />
    &nbsp; &nbsp; &nbsp;2821 ttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataaccc<br />
    &nbsp; &nbsp; &nbsp;2881 tcgagccacccatgaccaaaatcccttaacgtgagttacgcgtcgttccactgagcgtca<br />
    &nbsp; &nbsp; &nbsp;2941 gaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgc<br />
    &nbsp; &nbsp; &nbsp;3001 tgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagcta<br />
    &nbsp; &nbsp; &nbsp;3061 ccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtcctt<br />
    &nbsp; &nbsp; &nbsp;3121 ctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctc<br />
    &nbsp; &nbsp; &nbsp;3181 gctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccggg<br />
    &nbsp; &nbsp; &nbsp;3241 ttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcg<br />
    &nbsp; &nbsp; &nbsp;3301 tgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgag<br />
    &nbsp; &nbsp; &nbsp;3361 cattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggc<br />
    &nbsp; &nbsp; &nbsp;3421 agggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttat<br />
    &nbsp; &nbsp; &nbsp;3481 agtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggg<br />
    &nbsp; &nbsp; &nbsp;3541 gggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgc<br />
    &nbsp; &nbsp; &nbsp;3601 tggccttttgctcacatgtt<br />
    //<br />
    <br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><b><span style="font-family:&quot;微软雅黑&quot;,sans-serif">质粒菌株产品操作说明书</span></b></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">一、扩增流程</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="color:#333333">收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 1</span></span><span style="color:#333333">、质粒干粉（常温运输，存于</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">-20</span></span><span style="color:#333333">度，</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">90</span></span><span style="color:#333333">天保质期，请务必转化挑单克隆培养，不要直接使用和测序）</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="font-family:宋体"><span style="color:#333333">①</span></span><span style="color:#333333">收到质粒干粉后请先</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">5000rpm</span></span><span style="color:#333333">离心</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">1min</span></span><span style="color:#333333">，再加入</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">20&mu;l ddH2O</span></span><span style="color:#333333">去离子水溶解质粒；</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="font-family:宋体"><span style="color:#333333">②</span></span><span style="color:#333333">取</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">1</span></span><span style="color:#333333">支</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">100&mu;l </span></span><span style="color:#333333">感受态于冰上解冻</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">10min</span></span><span style="color:#333333">，加入</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">2&mu;l</span></span><span style="color:#333333">质粒，再冰浴</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">30min</span></span><span style="color:#333333">后，</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">42</span></span><span style="font-family:宋体"><span style="color:#333333">℃</span></span><span style="color:#333333">热激</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">60s</span></span><span style="color:#333333">，不要搅动，再冰浴</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">2min</span></span><span style="color:#333333">；（从第二步开始均要在超净工作台中无菌操作）</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="font-family:宋体"><span style="color:#333333">③</span></span><span style="color:#333333">加入</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">900&mu;l</span></span><span style="color:#333333">无抗的</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">LB</span></span><span style="color:#333333">液体培养基，</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">180rpm</span></span><span style="color:#333333">震荡</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">37</span></span><span style="font-family:宋体"><span style="color:#333333">℃</span></span><span style="color:#333333">培养</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">45min</span></span><span style="color:#333333">；</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="font-family:宋体"><span style="color:#333333">④</span></span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">6000rpm</span></span><span style="color:#333333">离心</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">5min</span></span><span style="color:#333333">，仅留</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">100&mu;l</span></span><span style="color:#333333">上清液重悬细菌沉淀，并涂布至目标质粒抗性的</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">LB</span></span><span style="color:#333333">平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="font-family:宋体"><span style="color:#333333">⑤</span></span><span style="color:#333333">将平板正向培养</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">1h</span></span><span style="color:#333333">，再倒置</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">37</span></span><span style="font-family:宋体"><span style="color:#333333">℃</span></span><span style="color:#333333">培养</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">14h</span></span><span style="color:#333333">。如果要求是</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">30</span></span><span style="color:#333333">度则培养</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">20h</span></span><span style="color:#333333">；</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">（菌落过多则将质粒稀释后再转化。没有菌落则加入</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">10&mu;l</span></span><span style="color:#333333">质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="font-family:宋体"><span style="color:#333333">⑥</span></span><span style="color:#333333">挑取单菌落至</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">LB</span></span><span style="color:#333333">液体培养基中，加入对应抗生素，</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">220rpm</span></span><span style="color:#333333">震荡培养</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">14h</span></span><span style="color:#333333">，根据实验需要和质粒提取试剂盒说明书提取质粒。</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 2</span></span><span style="color:#333333">、甘油菌种（冰袋运输，存于</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">-80</span></span><span style="font-family:宋体"><span style="color:#333333">℃</span></span><span style="color:#333333">，保质期</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">90</span></span><span style="color:#333333">天，请务必划线挑单克隆培养）</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<span style="color:#333333">四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 3</span></span><span style="color:#333333">、穿刺菌种（冰袋运输，存于</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">4</span></span><span style="font-family:宋体"><span style="color:#333333">℃</span></span><span style="color:#333333">，保质期</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">7</span></span><span style="color:#333333">天）</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="color:#333333">穿刺接种，液体培养后四区划线，再挑单菌落液体培养。</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 4</span></span><span style="color:#333333">、菌落平板（冰袋运输，存于</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">4</span></span><span style="font-family:宋体"><span style="color:#333333">℃</span></span><span style="color:#333333">，保质期</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">7</span></span><span style="color:#333333">天）</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="color:#333333">直接挑取单菌落至液体培养基中。</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 5</span></span><span style="color:#333333">、液体质粒（冰袋运输，存于</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">-20</span></span><span style="font-family:宋体"><span style="color:#333333">℃</span></span><span style="color:#333333">，保质期</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">90</span></span><span style="color:#333333">天）</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="color:#333333">单独提取的液体质粒收到后可直接使用。</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;6</span></span><span style="color:#333333">、滤纸质粒（常温运输，存于</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">-20</span></span><span style="color:#333333">度，</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">90</span></span><span style="color:#333333">天保质期，请务必转化挑单克隆培养，不要直接使用和测序）</span></span></span><br />
    <span style="font-size:10.5pt"><span style="font-family:等线">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <span style="color:#333333">收到货后将滤纸画圈部分剪下放入</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">EP</span></span><span style="color:#333333">管中，加</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">100ul</span></span><span style="color:#333333">无菌水将滤纸浸湿并浸泡</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">5min</span></span><span style="color:#333333">，吸取</span><span lang="EN-US" style="font-family:&quot;Arial&quot;,sans-serif"><span style="color:#333333">5ul</span></span><span style="color:#333333">质粒转化，离心全涂。</span></span></span><br />
    <br />
    <span style="font-size:10.5pt"><span style="font-family:等线"><span style="color:#333333">二、转化图片</span></span></span><br />
    <img alt="" src="https://img1.dxycdn.com/2021/0710/533/6058013828668851943-14.jpg" style="width: 400px; height: 389px;" /><br />
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    质粒菌株产品操作说明书
    一、扩增流程
             收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。
             1、质粒干粉（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
                  ①收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O去离子水溶解质粒；
                  ②取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；（从第二步开始均要在超净工作台中无菌操作）
                  ③加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min；
                  ④6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）
                  ⑤将平板正向培养1h，再倒置37℃培养14h。如果要求是30度则培养20h；
    （菌落过多则将质粒稀释后再转化。没有菌落则加入10μl质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）
                  ⑥挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
            2、甘油菌种（冰袋运输，存于-80℃，保质期90天，请务必划线挑单克隆培养）
                  四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。
            3、穿刺菌种（冰袋运输，存于4℃，保质期7天）
                  穿刺接种，液体培养后四区划线，再挑单菌落液体培养。
            4、菌落平板（冰袋运输，存于4℃，保质期7天）
                  直接挑取单菌落至液体培养基中。
            5、液体质粒（冰袋运输，存于-20℃，保质期90天）
                  单独提取的液体质粒收到后可直接使用。
            6、滤纸质粒（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
                  收到货后将滤纸画圈部分剪下放入EP管中，加100ul无菌水将滤纸浸湿并浸泡5min，吸取5ul质粒转化，离心全涂。

    二、转化图片
    产品细节图片2
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