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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
12个月
- 英文名:
Drop-n-Stain EverBrite™ Mounting Medium 23008
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
Drop-n-Stain EverBrite™ Mounting Medium is a wet-set antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. The dropper bottle makes for quick and easy dispensing.
- Convenient dropper bottle for pipette-free mounting
- Protects fluorescent dyes from rapid photobleaching
- Compatible with cyanine-based fluorophores like Cy®3, Cy®5, and Alexa Fluor® 647, unlike VECTASHIELD®
- Available with or without DAPI for nuclear counterstaining
- Works as well as original EverBrite™, but less viscous for rapid DAPI staining
Drop-n-Stain EverBrite™ Mounting Medium is a wet-set medium that remains liquid. After mounting, coverslips can be sealed with nail polish or Biotium’s CoverGrip™ Coverslip Sealant (23005). Mounted slides can be stored at 4°C or -20°C, protected from light. Antibody-stained slides can be preserved for a year or longer (note: some labels, like phalloidin, are less stable during storage). Drop-n-Stain EverBrite™ Mounting Medium has a refractive index of 1.42.
We also offer EverBrite™ Hardset Mounting Medium, which cures overnight to form a hard seal, eliminating the need to seal the edges of the coverslips.
EverBrite™ Mounting Media
| Product | Counterstain | Cat. No. |
|---|---|---|
| EverBrite™ Mounting Medium | None | 23001 |
| EverBrite™ Mounting Medium with DAPI | DAPI | 23002 |
| Drop-n-Stain EverBrite™ Mounting Medium | None | 23008 |
| Drop-n-Stain EverBrite™ Mounting Medium with DAPI | DAPI | 23009 |
| EverBrite™ Hardset Mounting Medium | None | 23003 |
| EverBrite™ Hardset Mounting Medium with DAPI | DAPI | 23004 |
| EverBrite™ Hardset with NucSpot® 640 | NucSpot® 640 | 23016 |
| EverBrite TrueBlack® Hardset Mounting Medium | None | 23017 |
| EverBrite TrueBlack® Hardset with DAPI | DAPI | 23018 |
| EverBrite TrueBlack® Hardset with NucSpot® 640 | NucSpot® 640 | 23019 |
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文献和实验Fluorescence Mounting Medium (Antifade)
should appear almost colorless to a slight tint of yellow. If it is an intense yellow or orange color the PPD is most likely contaminated and will have background staining. pH the mounting medium to a pH of 8.0-9.0 using the Carbonate-Bicarbonate buffer
Fluorescence Mounting Medium (Antifade)
to a slight tint of yellow. If it is an intense yellow or orange color the PPD is most likely contaminated and will have background staining. pH the mounting medium to a pH of 8.0-9.0 using the Carbonate-Bicarbonate buffer. pH paper of range 6.5-10.0
Rinse in distilled water for 5 min Stain in carmine alum O/N Wash in 70 % EtOH 15 min Wash in 95 % EtOH 15 min Wash in 100 % EtOH 15 min Clear in xylene and mount with Permount After photographic documentation the tissue












