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Drop-n-Stain EverBrite™ Mounti

ng Medium 23008
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  • 询价
  • Biotium已认证
  • 美国
  • 23008
  • 2026年01月23日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      -20°C

    • 保质期

      12个月

    • 英文名

      Drop-n-Stain EverBrite™ Mounting Medium 23008

    • 库存

      99

    • 供应商

      Biotium

    • CAS号

      1

    Drop-n-Stain EverBrite™ Mounting Medium is a wet-set antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes.  The dropper bottle makes for quick and easy dispensing.

    • Convenient dropper bottle for pipette-free mounting
    • Protects fluorescent dyes from rapid photobleaching
    • Compatible with cyanine-based fluorophores like Cy®3, Cy®5, and Alexa Fluor® 647, unlike VECTASHIELD®
    • Available with or without DAPI for nuclear counterstaining
    • Works as well as original EverBrite™, but less viscous for rapid DAPI staining

    Drop-n-Stain EverBrite™ Mounting Medium is a wet-set medium that remains liquid. After mounting, coverslips can be sealed with nail polish or Biotium’s CoverGrip™ Coverslip Sealant (23005). Mounted slides can be stored at 4°C or -20°C, protected from light. Antibody-stained slides can be preserved for a year or longer (note: some labels, like phalloidin, are less stable during storage). Drop-n-Stain EverBrite™ Mounting Medium has a refractive index of 1.42.

    Drop-n-Stain EverBrite FITC
    Photostability of FITC staining in HeLa cells with Drop-n-Stain EverBrite™ Mounting Medium compared to buffer alone.

    We also offer EverBrite™ Hardset Mounting Medium, which cures overnight to form a hard seal, eliminating the need to seal the edges of the coverslips.

    EverBrite™ Mounting Media

    Product Counterstain Cat. No.
    EverBrite™ Mounting Medium None 23001
    EverBrite™ Mounting Medium with DAPI DAPI 23002
    Drop-n-Stain EverBrite™ Mounting Medium None 23008
    Drop-n-Stain EverBrite™ Mounting Medium with DAPI DAPI 23009
    EverBrite™ Hardset Mounting Medium None 23003
    EverBrite™ Hardset Mounting Medium with DAPI DAPI 23004
    EverBrite™ Hardset with NucSpot® 640 NucSpot® 640 23016
    EverBrite TrueBlack® Hardset Mounting Medium None 23017
    EverBrite TrueBlack® Hardset with DAPI DAPI 23018
    EverBrite TrueBlack® Hardset with NucSpot® 640 NucSpot® 640 23019
    Alexa Fluor is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare; VECTASHIELD is a registered trademark of Vector Laboratories.
     

     

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    图标文献和实验
    相关实验
    • Fluorescence Mounting Medium (Antifade)

      should appear almost colorless to a slight tint of yellow. If it is an intense yellow or orange color the PPD is most likely contaminated and will have background staining. pH the mounting medium to a pH of 8.0-9.0 using the Carbonate-Bicarbonate buffer

    • Fluorescence Mounting Medium (Antifade)

      to a slight tint of yellow. If it is an intense yellow or orange color the PPD is most likely contaminated and will have background staining. pH the mounting medium to a pH of 8.0-9.0 using the Carbonate-Bicarbonate buffer. pH paper of range 6.5-10.0

    • whole mount preparations

      Rinse in distilled water for 5 min Stain in carmine alum O/N Wash in 70 % EtOH 15 min Wash in 95 % EtOH 15 min Wash in 100 % EtOH 15 min Clear in xylene and mount with Permount After photographic documentation the tissue

    图标技术资料

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    资料下载:

    PI-23008-23009.pdf 附 (下载 0 次)

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