万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
咨询
- 亚型:
IgG
- 形态:
液态
- 保存条件:
-20℃
- 克隆性:
单克隆
- 标记物:
无
- 适应物种:
无
- 保质期:
2年
- 抗原来源:
咨询
- 目录编号:
N
- 级别:
高纯
- 库存:
99
- 供应商:
云萃生物
- 宿主:
咨询
- 应用范围:
ELISA、WB、THC等
- 浓度:
咨询
- 靶点:
咨询
- 抗体英文名:
N
- 抗体名:
N
- 规格:
100ul
.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage
and purifi ed YFP-SUMO-1 and CFP-RanGAP1 as model substrates that are covalently coupled in the presence of recombinant SUMO E1 and E2 enzymes and ATP. Reactions of 25 μl volume, set up in 384-wells plates, give suffi cient signal for analysis. Consequently
Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
for in vitro sumoylation of RanGAP1 and Sp100 in the absence or presence of the SUMO E3 ligase RanBP2, respectively, are provided. Both protocols can be adapted easily to test in vitro conjugation of other target proteins and/or E3 ligases.








