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文献和实验Methods to Investigate the Role of SIRT1 in Endothelial Senescence
Sirtuins are a family of proteins with NAD+ -dependent deacetylase or mono-ADP-ribosyltransferase activity. SIRT1, the mammalian ortholog most closely related to Sir2 (the first gene of this family discovered in yeast), exhibits anti
SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome
, in combination with affinity enrichment of acetyllysine peptides and mass spectrometry, to study the dynamic changes of the Lysine acetylome in response to Sirt1. The method should be applicable to quantify changes to other post translational modifications
through mathematical modeling. We further illustrate the approach using as case study the signaling module composed by the proteins p53, Sirt1, and the regulatory miRNA miR-34a. The model was used not only to investigate different possible designs of the silencing
