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- 文献和实验
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- 英文名:
4-Bromo-2-ethoxy-1-nitrobenzene
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
57279-70-6
- 规格:
2mg/10mg/500mg/500g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 500g | 产品价格: | ¥100.0 |
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
5-bromo-4-chloro-3-indoyl phosphate/ ml of DMSO store at room temp in the dark (wrapped in foil) Sigma B8503 nBT - 33 mg nitroblue tetrazolium/ml of 70% DMSO store at room temp in the dark (wrapped in foil) Sigma N6876 mix
with strain #95. Grow strains overnight with antibiotic selection (Tet3 for #95). Subculture 1:100 in fresh medium (no antibiotics). Grow at 37o C to early log phase (when cell swirls are visible). The recipient strain (B211






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