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文献和实验4.5 µL 4 M NH2 OH 6. Incubate at r.t. in dark for 15 min. E. Cleanup in QiaQuick columns 1. Combine Cy5 and Cy3 samples (experiment and control). 2. Add: 70 µL H2 O, 500 µL Buffer PB 3. Apply to a QiaQuick column. Spin 13K g for 30".
Amino-allyl Reverse Transcription
Resuspend the contents of one pack of the monofunctional NHS-ester Cy3 or Cy5 dye (mono-functional Cy3 or Cy5 reactive pack, Amersham catalog PA 23001 and PA 25001) in 72 ul DMSO. Aliquot 16 x 4.5 ul and either use immediately or dry
Amino-allyl Reverse Transcription
Amino-allyl Reverse Transcription (this protocol was adapted by Joe DeRisi from one developed at Rosetta Inpharmatics) Resuspend the contents of one pack of the monofunctional NHS-ester Cy3 or Cy5 dye (mono-functional Cy3 or Cy5
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