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文献和实验Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab)2, and Streptavidin
Colloidal gold probes, including protein A-, IgG-F(ab’ )2 -, and streptavidin-labeled gold particles, are useful tools for localization of antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different
DNA Extraction of Ancient Animal Hard Tissue Samples via Adsorption to Silica Particles
in these specimens, an efficient DNA release and purification method is required. In this chapter, I describe an efficient and straightforward purification and concentration method that uses DNA adsorption to a solid surface of silica particles. Comparative analysis
Labeled Sphingomyelin Synthesis
. Column purification of labeled SM: 12) Pack a Bio-Sil A column as follows: a) Pack base of column with a small portion of glass wool. b) Suspend 20 grams of 100-200 mesh BioSil A in 100 ml chloroform. c) Pour silica/chloroform mixture
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