WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-800 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
14/16kDa
细胞定位
细胞浆 细胞膜
性 状
Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human LC3A:1-100/121
亚 型
IgG
纯化方法
affinity purified by Protein G
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Microtubule-associated MAPILC3A constitutes nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. MAP1LC3A is one of three human orthologs of the rat Map1LC3, (named MAP1LC3A, MAP1LC3B, and MAP1LC3C). The three isoforms of human MAP1LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue; MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.
Function: Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Subunit: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Interacts with SQSTM1. Interacts with TP53INP1 and TP53INP2.
Tissue Specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
Post-translational modifications: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Similarity: Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).