;THIF; thioredoxin binding protein 2; thioredoxin interacting protein; Thioredoxin-binding protein 2; Thioredoxin-interacting protein; upregulated by 1,25-dihydroxyvitamin D-3; VDUP1; Vitamin D3 up-regulated protein 1; TXNIP_HUMAN.
研究领域
肿瘤 免疫学 转录调节因子
抗体来源
Rabbit
克隆类型
Polyclonal
交叉反应
Human, Rat, (predicted: Mouse, Dog, Pig, Rabbit, )
产品应用
ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量
44kDa
细胞定位
细胞核 细胞浆
性 状
Liquid
浓 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human TXNIP:101-200/391
亚 型
IgG
纯化方法
affinity purified by Protein A
储 存 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
TXNIP may act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. It interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. It functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. It is required for the maturation of natural killer cells (from SwissProt).
Function: May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).
Subunit: Interacts with TXN/thioredoxin through its redox-active site. Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1. Interacts (via C-terminus) with ITCH (via WW domains). Interacts with DDIT4.
Subcellular Location: Cytoplasm
Post-translational modifications: Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.
Similarity: Belongs to the arrestin family.
SWISS: Q9H3M7
Gene ID: 10628
Database links: UniProtKB/Swiss-Prot: Q9H3M7.1
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
硫氧还蛋白结合蛋白-2 (TRX-binding protein-2,TBP-2)又称:维生素D3上调蛋白1(Vitamin D3 up-regulated protein 1,VDUP1)或称硫氧还蛋白相互作用蛋白(Thioredoxin-interacting protein,Txnip)。 TBP-2与硫氧还蛋白(thioredoxin,TRX)的活性部位结合,抑制其活性,除了参与多种氧化应激反应,还具有多种生物学功能。 有学者认为:TBP-2与肿瘤密切相关,有些肿瘤组织中,TBP-2表达降低或缺失。TBP-2表达增高时,细胞周期停滞,肿瘤细胞繁殖受到抑制。目前已成为肿瘤研究的重要靶点。