- ￥1200
- Biorigin
- BN41524R
- 2025年08月09日
- FCM,
- Human,Mouse,Rat,Dog,Cow,Sheep,Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 适应物种：
Human,Mouse,Rat,Dog,Cow,Sheep,Guinea Pig,
- 应用范围：
FCM,
- 抗体英文名：
IASPP
- 规格：
50ul
| 英文名称 | IASPP |
| 中文名称 | 凋亡抑制因子IASPP抗体 |
| 别 名 | IASPP; inhibitor of apoptosis stimulating protein of p53; Inhibitor of ASPP protein; NFkB interacting protein 1; NKIP1; PPP1R13B-like protein; PPP1R13L; Protein iASPP; protein phosphatase 1 regulatory (inhibitor) subunit 13 like; RAI; RelA-associated inhibitor; IASPP_HUMAN; RelA-associated inhibitor; Inhibitor of ASPP protein; NFkB-interacting protein 1. |
| 研究领域 | 肿瘤 免疫学 染色质和核信号 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, (predicted: Mouse, Dog, Cow, Sheep, Guinea Pig, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 89kDa |
| 细胞定位 | 细胞核 细胞浆 细胞外基质 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IASPP:15-100/828 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | iASPP(inhibitor of apoptosis stimulating protein of p53)regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. Highly expressed in heart, placenta and prostate. Weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. Belongs to the ASPP family. Function: Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. Subunit: Interacts with RELA NF-kappa-B subunit and with SP1 via its C-terminus part. Interacts with p53/TP53, TP63 and TP73. Subcellular Location: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic but also nuclear. Tissue Specificity: Highly expressed in heart, placenta and prostate. Weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. Similarity: Belongs to the ASPP family. Contains 2 ANK repeats. Contains 1 SH3 domain. SWISS: Q8WUF5 Gene ID: 10848 Database links: Entrez Gene: 10848 Human Entrez Gene: 333654 Mouse Omim: 607463 Human SwissProt: Q8WUF5 Human SwissProt: Q3TCU2 Mouse SwissProt: Q5I1X5 Mouse Unigene: 466937 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. IASPP(inhibitory member of the ASPP family) 是新近发现的高度保守的p53相关基因, 是细胞凋亡ASPP家族的另一个成员。 |
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文献和实验【求助】请问western blot做凋亡检测时，识别caspase-3酶原和激活的caspase-3的抗体是相同的吗？
lsdcfhyj 以及检测PARP的裂解前后蛋白水平用的抗体是否都是一样的？ edwardellen 有两者都识别的，也有专门识别剪切体不识别酶原的，用过cell signal的，两种都很好用。 PARP也有可以同时识别全长和剪切体的抗体。 magichunter 检测caspase-3和激活型的caspase-3分别需要两种不同的抗体，一般CST公司卖的分别包括：cleaved
【摘要】目的探讨嵌合抗CD20抗体Fab’片段的抗肿瘤机制。方法应用MTT法检测Fab’片段对Raji细胞生长的抑制作用，用Annexin V-FITC和PI测定Fab’片段对Raji细胞凋亡的诱导作用，用RT-PCR和Western blot检测Raji细胞中bcl-2表达的变化。结果嵌合抗CD20Fab’片段对Raji细胞具有明显的抑制作用IC50值为24.2 µg/ml，并呈剂量依赖性；嵌合抗CD20Fab’片段能诱导Raji细胞的凋亡，并降低Raji细胞中bcl-2基因及其蛋白的表达
单链抗体可变区基因片段五聚体作用于凋亡诱导配体受体-2来增强其对癌症的治疗作用
一种有效的途径来延长它在血中的半衰期。这里有好几种方法可以用来生成多聚的scFvs。例如，两个或更多的scFvs已经通过改变重链可变区和轻链可变区的连接肽的长度被组合成二聚体或三聚体，并且多个scFv分子已经通过化学交联被结合在了一起，例如聚乙二醇化。此外，scFvs的多聚体已经通过与多聚的结构域相结合而被合成，这些结构域包括Fc、亮氨酸拉链结构等。最近的一篇论文指出scFv-Fc-scFv双特异性分子可以导致半衰期约460h的抗体样清除。作为一个参与细胞凋亡的关键分子，肿瘤坏死因子相关凋亡
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