4nm Colloidal Gold-AffiniPure

免疫胶体金(Immune colloidal gold)

金蛋白结合物装入透析袋，在硅胶中脱水浓缩至原体积的 1 ／ 5 ～ 1 ／ 10 。再经 1 500r/min 离心 20min 。取上清加至 Sephacryl S-400 （丙烯葡聚糖凝胶 S-400 ）层析柱分别纯化。层析柱为 0.8 cm × 20cm ，加样量为床体积的 1 ／ 10 ，以 0.02Mol/L PBS 液洗脱（内含 0.1 ％ BSA ， 0.05 ％ NaN3 ， pH8.2 者用 IgG 标记物），流速为 8ml/h 。按红色深浅分管

Silver Enhancement of Colloidal Gold

. for 1-nm gold, for Nanogold 4 to 5 min (20 - 22°C). The final pH is 4 (see Burry 1995, p. 220). Remove blocking buffer, add 25 µl of the primary antibody solution per coverslip (with a final concentration in the range of 1-5 µg specific IgG/ml) and incubate

E L I S A w i t h p l a t e l e t s

for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS