组蛋白H2B单克隆抗体

英文名称Histone H2B中文名称组蛋白H2B单克隆抗体别    名H2B.1 A; H2B/a; H2B/g; H2B/h; H2B/k; H2B/l; H2B/l.  研究领域细胞生物  表观遗传学  抗体来源Mouse克隆类型Monoclonal克 隆 号4H9交叉反应Human, Mouse, Rat, 产品应用IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.分 子 量14kDa细胞定位细胞核 性    状Liquid浓    度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human Histone H2B: 亚    型IgG纯化方法affinity purified by Protein G储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed产品介绍Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008]

Function:
In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA, making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. Whilst the core histones are highly conserved across a wide range of organisms, the linker histones are less conserved.

Subcellular Location:
Nuclear

Similarity:
Belongs to the histone H2B family.

SWISS:
P33778

Gene ID:
3017

Database links:

 

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.