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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
MC3T3-E1 Subclone 14
- 库存:
1x10^6/cell
- 供应商:
上海酶研
- 肿瘤类型:
无
- 细胞类型:
MC3T3-E1 Subclone 14
- ATCC Number:
详询
- 品系:
MC3T3-E1 Subclone 14
- 组织来源:
MC3T3-E1 Subclone 14[小鼠颅顶前骨细胞亚克隆14]
- 相关疾病:
//
- 物种来源:
小鼠
- 免疫类型:
1
- 细胞形态:
正常
- 是否是肿瘤细胞:
否
- 器官来源:
MC3T3-E1 Subclone 14[小鼠颅顶前骨细胞亚克隆14]
- 运输方式:
自取或顺丰快递发货
- 年限:
永久
- 生长状态:
活力好,代次低
- 规格:
T25
Cell line name MC3T3-E1 Subclone 14
Synonyms MC3T3-E1 SUBCLONE 14
Accession CVCL_5437
Resource Identification Initiative To cite this cell line use: MC3T3-E1 Subclone 14 (RRID:CVCL_5437)
Comments Characteristics: Exhibits high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. Forms a well mineralized extracellular matrix (ECM) after 10 days (ATCC=CRL-2594).
Derived from site: In situ; Bone, calvaria; UBERON=UBERON_0004339.
Cell type: Osteoblast; CL=CL_0000062.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Publications
PubMed=10352097; DOI=10.1359/jbmr.1999.14.6.893
Wang D., Christensen K., Chawla K., Xiao G.-Z., Krebsbach P.H., Franceschi R.T.
Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential.
J. Bone Miner. Res. 14:893-903(1999)
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文献和实验*发表【中文论文】请标注:由上海酶研生物科技有限公司提供;
*发表【英文论文】请标注:From Shanghai EK-Bioscience Biotechnology Co., Ltd.
(such as AluI digested cosmid). E. Competent cell preparation For preparation of competent bacterial cells (14), a glycerol cell culture stock of the respective E. coli strain is thawed and added to 50 ml of liquid media. This culture then is preincubated
(such as AluI digested cosmid). E. Competent cell preparation For preparation of competent bacterial cells (14), a glycerol cell culture stock of the respective E. coli strain is thawed and added to 50 ml of liquid media. This culture then is preincubated
问:BAF cell的来源是什么?有什么特性和用途呢?答:ATCC上没有你所谓的BAF cell,DSMZ上有两种BAF cell,如下:Cell line:BA-F8Cell type:mouse hybridoma(anti-rat fiber/myosin)Origin:this hybridoma cell line was described to secrete a mouse monoclonal antibody(IgG2b)that reacts with rat type
技术资料







