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- 英文名：
SU-DHL-10
- 库存：
1x10^6/瓶
- 供应商：
上海酶研
- 肿瘤类型：
淋巴瘤
- 细胞类型：
SU-DHL-10
- 品系：
SU-DHL-10
- 组织来源：
人大细胞淋巴瘤
- 相关疾病：
详询
- 物种来源：
人
- 免疫类型：
详询
- 细胞形态：
贴壁/悬浮
- 是否是肿瘤细胞：
是
- 器官来源：
人大细胞淋巴瘤
- 运输方式：
顺丰快递
- 年限：
5年
- 生长状态：
生长良好
- 规格：
瓶
SU-DHL-10细胞系、SU-DHL-10细胞株、SU-DHL-10细胞、SU-DHL-10细胞、SU-DHL-10 大细胞淋巴瘤
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: MD Anderson Cell Lines Project.
Population: Caucasian.
Doubling time: ~15-30 hours (DSMZ=ACC-576).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: miRNA expression profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Omics: Virome analysis using RNAseq.
Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
PubMed=8547074; DOI=10.1111/j.1365-2141.1995.tb05302.x
Siebert R., Willers C.P., Schramm A., Fossa A., Dresen I.M.G., Uppenkamp M.J., Nowrousian M.R., Seeber S., Opalka B.
Homozygous loss of the MTS1/p16 and MTS2/p15 genes in lymphoma and lymphoblastic leukaemia cell lines.
Br. J. Haematol. 91:350-354(1995)
PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x; PMCID=PMC5921886
Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)
PubMed=9787181; DOI=10.1182/blood.V92.9.3410
Sakai A., Thieblemont C., Wellmann A., Jaffe E.S., Raffeld M.
PTEN gene alterations in lymphoid neoplasms.
Blood 92:3410-3415(1998)
DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)
PubMed=19278952; DOI=10.1182/blood-2009-01-202028; PMCID=PMC3401058
Li C., Kim S.-W., Rai D., Bolla A.R., Adhvaryu S., Kinney M.C., Robetorye R.S., Aguiar R.C.T.
Copy number abnormalities, MYC activity, and the genetic fingerprint of normal B cells mechanistically define the microRNA profile of diffuse large B-cell lymphoma.
Blood 113:6681-6690(2009)
PubMed=19358282; DOI=10.1002/ijc.24351
Inagaki A., Ishida T., Yano H., Ishii T., Kusumoto S., Ito A., Ri M., Mori F., Ding J.-M., Komatsu H., Iida S., Ueda R.
Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
Int. J. Cancer 125:212-221(2009)
PubMed=20054396; DOI=10.1038/nature08638; PMCID=PMC2845535
Davis R.E., Ngo V.N., Lenz G., Tolar P., Young R.M., Romesser P.B., Kohlhammer H., Lamy L., Zhao H., Yang Y.-D., Xu W.-H., Shaffer A.L. 3rd, Wright G., Xiao W.-M., Powell J.I., Jiang J.-K., Thomas C.J., Rosenwald A., Ott G., Muller-Hermelink H.-K., Gascoyne R.D., Connors J.M., Johnson N.A., Rimsza L.M., Campo E., Jaffe E.S., Wilson W.H., Delabie J., Smeland E.B., Fisher R.I., Braziel R.M., Tubbs R.R., Cook J.R., Weisenburger D.D., Chan W.-C., Pierce S.K., Staudt L.M.
Chronic active B-cell-receptor signalling in diffuse large B-cell lymphoma.
Nature 463:88-92(2010)
PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)
PubMed=20628145; DOI=10.1182/blood-2010-05-282780; PMCID=PMC2995356
Green M.R., Monti S., Rodig S.J., Juszczynski P., Currie T., O'Donnell E., Chapuy B., Takeyama K., Neuberg D., Golub T.R., Kutok J.L., Shipp M.A.
Integrative analysis reveals selective 9p24.1 amplification, increased PD-1 ligand expression, and further induction via JAK2 in nodular sclerosing Hodgkin lymphoma and primary mediastinal large B-cell lymphoma.
Blood 116:3268-3277(2010)
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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
三句话读懂一篇 CNS：晚睡晚起导致抑郁；吃药代替运动健身；高脂肪饮食引发结直肠癌...
by LACE-seq。该研究首次在单细胞和单碱基分辨率层面精准鉴定 RBP 的结合位点，为研究 RBP 在生殖疾病和胚胎发育中的功能机制提供了理论依据。图 10：来源站酷海洛 Plus本周推荐：三句话读懂一篇 CNS：男性的大脑和睾丸相似；父亲熬夜，影响后代健康...参考文献：Jessica O’Loughlin et al.Using Mendelian Randomisation methods to understand whether diurnal preference is causally
使用BrU抗体，操作繁琐，干细胞或神经元细胞不摄取BrU，RNA input量高； 4sU-seq 优点：无需抗体，通过生物素标记和链霉亲和素磁珠进行可逆结合捕获4sU标记的RNA； 缺点：结合的效率会影响RNA降解速率的准确性 瞬时转录组测序（TT-seq） 优点：不能均一覆盖整个转录本，只有新转录本的3’端被标记上，所以需要去除5’端RNA； 缺点：标记时间短，TT-seq
上清液，加入二甲基亚砜（DMSO）100μl，平板床振荡10min。在酶联免疫检测仪上测定细胞的吸光度（OD）值，所用实验波长为490nm。将RFPSU2组与未进行RFP 转染的单纯SU2 生长对照组于转染后每日按上述步骤进行MTT 检测。以时间为横轴，OD 值为纵轴, 绘制两组细胞的生长曲线。 （5）慢病毒转染后脑胶质瘤干细胞的免疫荧光染色 取经多次体外连续传代培养慢病毒转染后的脑胶质瘤干细胞（RFP-SU2）行CD133、Nestin 免疫荧光染色。具体步骤：将RFP-SU2 种植
技术资料
