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- 技术资料
- 英文名：
MKN7
- 库存：
1x10^6/瓶
- 供应商：
上海酶研
- 肿瘤类型：
胃癌
- 细胞类型：
MKN7
- 品系：
MKN7
- 组织来源：
人胃癌细胞
- 相关疾病：
详询
- 物种来源：
人
- 免疫类型：
详询
- 细胞形态：
贴壁/悬浮
- 是否是肿瘤细胞：
是
- 器官来源：
人胃癌细胞
- 运输方式：
顺丰快递
- 年限：
5年
- 生长状态：
生长良好
MKN7、MKN-7、MKN7细胞、MKN-7细胞、MKN7人胃癌细胞
Cell line name MKN7
Synonyms MKN-7; MKN 7
Accession CVCL_1417
Resource Identification Initiative To cite this cell line use: MKN7 (RRID:CVCL_1417)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: JFCR39 cancer cell line panel.
Part of: JFCR45 cancer cell line panel.
Population: Japanese.
Doubling time: 64-66 hours (PubMed=3962675); 66 hours (PubMed=29435981); 86 hours (PubMed=25984343).
Microsatellite instability: Instable (MSI-low) (Sanger).
Omics: Array-based CGH.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Caution: TP53 mutation indicated incorrectly as being at p.Ile251Leu (c.751A>C) in PubMed=15900046.
Derived from site: Metastatic; Lymph node; UBERON=UBERON_0000029.
Sequence variations
Mutation; HGNC; 11998; TP53; Simple; p.Pro278Ser (c.832C>T); ClinVar=VCV000376642; Zygosity=Homozygous (PubMed=11107048; Cosmic-CLP=924250; DepMap=ACH-000678).
HLA typing Source: PubMed=26589293
Class I
HLA-A A*24:02,24:02
HLA-B B*07:02,48:01
HLA-C C*04:01,08:03
Genome ancestry Source: PubMed=30894373
Origin % genome
African 0
Native American 0.09
East Asian, North 81.05
East Asian, South 18.37
South Asian 0
European, North 0
European, South 0.49
Disease Gastric tubular adenocarcinoma (NCIt: C5473)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_4Y05 (MKN7/CMV-Luc)
Sex of cell Male
Age at sampling 39Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=924250; JCRB=JCRB1025; PubMed=25877200; RCB=RCB0999; TKG=TKG 0228
Markers:
Amelogenin X
CSF1PO 10,13
D3S1358 17
D5S818 9,13
D7S820 9 (JCRB=JCRB1025; PubMed=25877200; TKG=TKG 0228)
9,11 (Cosmic-CLP=924250; RCB=RCB0999)
D8S1179 15
D13S317 8,12
D16S539 9,10
D18S51 13,18
D21S11 29,32.2
FGA 23
Penta D 10,11
Penta E 18
TH01 7,9
TPOX 9
vWA 17
PubMed=1933850
Yamada Y., Yoshida T., Hayashi K., Sekiya T., Yokota J., Hirohashi S., Nakatani K., Nakano H., Sugimura T., Terada M.
p53 gene mutations in gastric cancer metastases and in gastric cancer cell lines derived from metastases.
Cancer Res. 51:5800-5805(1991)
DOI=10.1016/B978-0-12-333530-2.50014-9
Sekiguchi M., Suzuki T.
Gastric tumor cell lines.
(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.287-316; Academic Press; New York; USA (1994)
PubMed=11107048; DOI=10.1046/j.1440-1827.2000.01117.x
Yokozaki H.
Molecular characteristics of eight gastric cancer cell lines established in Japan.
Pathol. Int. 50:767-777(2000)
PubMed=15723654; DOI=10.1111/j.1349-7006.2005.00016.x; PMCID=PMC11160020
Takada H., Imoto I., Tsuda H., Sonoda I., Ichikura T., Mochizuki H., Okanoue T., Inazawa J.
Screening of DNA copy-number aberrations in gastric cancer cell lines by array-based comparative genomic hybridization.
Cancer Sci. 96:100-110(2005)
PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
Nakatsu N., Yoshida Y., Yamazaki K., Nakamura T., Dan S., Fukui Y., Yamori T.
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Mol. Cancer Ther. 4:399-412(2005)
PubMed=15900046; DOI=10.1093/jnci/dji133
Mashima T., Oh-hara T., Sato S., Mochizuki M., Sugimoto Y., Yamazaki K., Hamada J.-i., Tada M., Moriuchi T., Ishikawa Y., Kato Y., Tomoda H., Yamori T., Tsuruo T.
p53-defective tumors with a functional apoptosome-mediated pathway: a new therapeutic target.
J. Natl. Cancer Inst. 97:765-777(2005)
PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)
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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
所需的时间就比夏天要长的多。SGC-7901胃癌细胞的消化传代大致步骤同上，但胰酶作用的时候应放在培养箱中，夏天0.1%的胰酶消化2分钟左右就够了，但冬天差不多要8分钟左右MDA-MB-231乳腺癌细胞消化传代基本同MKN-28，只是消化时间可稍长一点，1分钟左右。
本人养胃癌细胞sgc-7901已经半年多了，这半年来，可以说是及其不顺，同门的师哥师姐都说这个细胞很好养，增殖快，传代快，生命力旺盛，不容易感染，然，到我这里似乎就不像他们说的那样，问题接二连三的出现，但我从这一连串的失败中从一个懵懂无知者变成一个动作娴熟的能手，说句实在话还得感谢这批细胞给我带来的宝贵经验。首先我要说一点：无菌操作刚一接手细胞时，无菌相关事项早已耳熟能详，但我的第一批细胞在传第二代时不幸夭折，不明原因，总结一下还应该是操作的问题，被感染，虽没做细菌培养，但还应该是被感染致死
0.3% TE13* 0.3% 胃癌 (1.45±0.84%) NUGC3 2.1% MKN1 2.2% MKN7 0.6% MKN28* 0.8% 结肠癌 (0.43±0.07%) WiDr 0.3% CCK81* 0.5% Colo201 0.3% Colo205 0.5% SW480** 0.7% HSC15 0.3% 肝癌 (0.90±0.90%) HuH7** 0.9% Hep3B* 1.8% HepG2 0.0 胰腺癌
技术资料