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- 库存：
大量
- 英文名：
MycoLight™ Rapid Fluorescence Gram-Positive Bacteria Staining Kit
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
100 Tests
关键词：MycoLight 快速荧光革兰氏阳性细菌染色试剂盒详细如下
AAT Bioquest's MycoLight™ Rapid Fluorescence Gram-Positive Bacteria Staining Kit provides a novel one-step fluorescence assay for the determination of gram sign in living bacteria. The gram stain is an important and widely used method for the taxonomic classification of bacteria in clinical and research settings. The original method involves quite a few steps like heat fixation, two-steps staining protocol, alcohol extraction and counterstaining. These steps can create inconsistent staining. AAT Bioquest's one-step kit overcomes the existing problems by eliminating the labor-intensive steps. The kit uses a fluorescently labeled Concanavalin A (ConA), which is a lectin that selectively binds to N-acetyl glucosamine exposed on the surface of gram-positive bacteria. When gram-negative and gram-positive bacteria are stained with the fluorescently labeled ConA conjugate, only gram-positive bacteria fluoresce red. Stained bacteria can be monitored fluorimeterically. Our kit is robust and convenient since the fluorescently labeled ConA conjugate used in our kit demonstrates higher brightness and photo stability over other existing dyes.
Components
Component A: IF647-ConA 1 vial
Component B: Assay Buffer 1 bottle (25 mL)
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-104550P0 | iFluor™ 820 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550P1 | iFluor™ 820 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550Q0 | iFluor™ 840 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550Q1 | iFluor™ 840 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550R0 | iFluor™ 860 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550R1 | iFluor™ 860 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550S0 | iFluor™ A7 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550S1 | iFluor™ A7 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550T0 | mFluor™ Blue 570 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550T1 | mFluor™ Blue 570 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550U0 | mFluor™ Green 620 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550U1 | mFluor™ Green 620 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550V0 | mFluor™ Red 700 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550V1 | mFluor™ Red 700 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550W0 | mFluor™ Red 780 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550W1 | mFluor™ Red 780 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550X0 | mFluor™ UV375 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550X1 | mFluor™ UV375 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550Y0 | mFluor™ UV460 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550Y1 | mFluor™ UV460 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-104550Z0 | mFluor™ Violet 450 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-104550Z1 | mFluor™ Violet 450 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-10455100 | mFluor™ Violet 500 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-10455101 | mFluor™ Violet 500 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-10455110 | mFluor™ Violet 510 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-10455111 | mFluor™ Violet 510 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-10455120 | mFluor™ Violet 540 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-10455121 | mFluor™ Violet 540 Anti-human CD45 Antibody *HI100* | 500tests |
| AAT-10455140 | AF350 Anti-human CD45 Antibody *HI100* | 100tests |
| AAT-10455141 | AF350 Anti-human CD45 Antibody *HI100* | 500tests |
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文献和实验色，为绿脓菌素试验阳性，否则为阴性。本菌为绿脓菌素试验阳性。 荧光试验：接种于金氏B 培养基(或假单胞菌产荧光色素培养基) 上，36℃±1℃ 培养18-24 小时，在366nm 紫色灯下有蓝绿色荧光。 2.4 枯草芽孢杆菌： 菌落形态：表面粗糙不透明，有皱摺。 镜检：用芽孢染色液染色，菌体有芽孢。 2.5 白色念珠菌： 革兰氏染色试验：呈卵圆形，很象酵母菌，比葡萄球菌大5～6 倍，革兰氏染色阳性，但着色不均匀。 念球菌显色培养基：30-35℃ 培养24-48 小时，呈绿色菌落
中是否含有逆转录/扩增抑制剂（图 2）。 表 1：阳性内对照和相关试剂盒 图 2：通过 IPC 提示体系中抑制剂的存在。实验结果为同样浓度的 RNA 加入相同拷贝 Xeno IPC，在不同浓度的扩增抑制剂血红素存在下（0-4μM），Xeno IPC 的 Ct 值与目标 RNA 的 Ct 值定量结果。 阳性样本对照 Positive Sample Control 阳性内对照虽然可以在一定程度上反应核酸提取效率，但是却很难反馈提取流程中对核酸释放的效率。一些病原体的样本，尤其是含有革兰氏阳性
，以扣除自发荧光对其它通道的干扰。 Q：染色完不能及时上机，样本能否固定？ A：细胞染色完成后，禁止固定，尽快检测，如无法立即检测，可将样本管放冰上，以减少外界环境对检测结果的影响，Annexin V 结合不稳定，请务必在标记结束后半小时内检测。 如果实验室距离上机的地方较远，建议将细胞和试剂盒带到上机的地方，染色后立即检测。 Q：凋亡检测中如何设置对照组？凋亡检测时细胞全部检测都是阳性是什么原因？ A：Annexin V 凋亡检测中，阴阳性的界定不是依据空白组（细胞中不加 PI 也不加
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