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- 详细信息
- 文献和实验
- 技术资料
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- 英文名:
5-Bromo-2-ethylbenzofuran
- 库存:
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- 供应商:
无锡云萃生物
- CAS号:
39178-60-4
- 规格:
2mg/10mg/500mg/676g
| 规格: | 2mg | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥100.0 |
| 规格: | 500mg | 产品价格: | ¥100.0 |
| 规格: | 676g | 产品价格: | ¥276.0 |
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文献和实验. Perform PCR with a suitable program for the template, primers, and thermal cycler: 5 minutes at 95°C (especially important if using colony pick or unopened cells af any form) 30 times: 30 seconds at 95°
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
). However, for localization of cells containing transduced lacZ, chromogenic substrates that yield a precipitated product are desirable (Holt and O'Sullivan, 1958, Pearse 1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro










