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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
LysoBrite™ Green
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
500 Tests
关键词：LysoBrite 溶酶体绿色荧光探针详细如下
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. LysoBrite™ Green selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
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| AAT-100330O1 | iFluor™ 810 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330P0 | iFluor™ 820 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330P1 | iFluor™ 820 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330Q0 | iFluor™ 840 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330Q1 | iFluor™ 840 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330R0 | iFluor™ 860 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330R1 | iFluor™ 860 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330S0 | iFluor™ A7 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330S1 | iFluor™ A7 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330T0 | mFluor™ Blue 570 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330T1 | mFluor™ Blue 570 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330U0 | mFluor™ Green 620 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330U1 | mFluor™ Green 620 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330V0 | mFluor™ Red 700 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330V1 | mFluor™ Red 700 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330W0 | mFluor™ Red 780 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330W1 | mFluor™ Red 780 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330X0 | mFluor™ UV375 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330X1 | mFluor™ UV375 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330Y0 | mFluor™ UV460 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330Y1 | mFluor™ UV460 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-100330Z0 | mFluor™ Violet 450 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-100330Z1 | mFluor™ Violet 450 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-10033100 | mFluor™ Violet 500 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-10033101 | mFluor™ Violet 500 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-10033110 | mFluor™ Violet 510 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-10033111 | mFluor™ Violet 510 Anti-human CD3 Antibody *SK7* | 500tests |
| AAT-10033120 | mFluor™ Violet 540 Anti-human CD3 Antibody *SK7* | 100tests |
| AAT-10033121 | mFluor™ Violet 540 Anti-human CD3 Antibody *SK7* | 500tests |
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文献和实验，内含胞浆成分，如线粒体、内质网、核糖体等。 自噬溶酶体（AV2）的特征为：单层膜，胞浆成分已降解。（autophagic vacuole，AV） 2. 在荧光显微镜下采用 GFP-LC3 融合蛋白来示踪自噬形成 由于电镜耗时长，不利于监测（Monitoring）自噬形成，人们利用 LC3 在自噬形成过程中发生聚集的现象开发出了此技术。无自噬时，GFP-LC3 融合蛋白弥散在胞浆中；自噬形成时，GFP-LC3 融合蛋白转位至自噬体膜，在荧光显微镜下形成多个明亮的绿色荧光斑点，一个斑
主要分为两大类，第一类是 PKH67（绿色荧光）/PKH26（红色荧光），由于它们可以与外泌体的脂质双层膜稳定结合，所以染色效果较好，应用较广泛。 图1 PKH67 标记的外泌体与神经元之间相互作用 Acta Neuropathol Commun. 2018 Feb 15;6(1):10. 图2 PKH26 标记的外泌体与 MDA‐MB‐231 细胞共培养 Cancer Sci. 2019 Oct;110(10):3173-3182. 第二类是 Di 系列的亲脂性染料，包括DiI（橙色荧
一、线粒体膜电位检测原理 线粒体膜电位下降是细胞凋亡早期的标志性事件，发生在细胞核凋亡特征（染色质浓缩、DNA断裂）出现之前，一旦线粒体膜电位崩溃，细胞凋亡便不可逆转。 JC-1是一种广泛用于检测线粒体膜电位∆Ψm的理想荧光探针，可以检测细胞、组织或纯化的线粒体膜电位。JC-1有单体和多聚体两种存在状态，两者发射光谱不同。在正常细胞内，线粒体膜电位较高，JC-1聚集在线粒体的基质中，形成聚合物，可产生红色荧光；凋亡早期，线粒体膜电位降低，JC-1不能聚集在线粒体基质中，此时JC-1为单体
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