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- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Cell Navigator™ Live Cell Endoplasmic Reticulum (ER) Staining Kit
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
100 Tests
关键词：Cell Navigator 活细胞内质网染色试剂盒 绿色荧光详细如下
The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae. The membranes of the ER are continuous with the outer nuclear membrane. ER occurs in most types of eukaryotic cells, but is absent from red blood cells and spermatozoa. This Cell Navigator™ Live Cell Endoplasmic Reticulum (ER) Staining Kit uses our ER Tracer™ Green as an ER marker. ER Tracer™ Green stain is a cell-permeant fluorescent dye that is highly selective for ER. This stain consists of a green fluorescent dye and ER binder that selectively bind to ER in most of cell types. For some cells, ER Tracer™ Green may not selectively bind to ER. ER Tracer™ Green has spectral properties essentially identical to FITC, making this kit convenient with the FITC filter set.
Components
Component A: ER Tracer™ Green 1 vial
Component B: Live Cell Staining Buffer 1 bottle (20 mL)
Component C: DMSO 1 vial (100 µL)
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-10032110 | mFluor™ Violet 510 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-10032111 | mFluor™ Violet 510 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-10032120 | mFluor™ Violet 540 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-10032121 | mFluor™ Violet 540 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-10032140 | AF350 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-10032141 | AF350 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-10032150 | AF488 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-10032151 | AF488 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-10032160 | AF555 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-10032161 | AF555 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-10032170 | AF594 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-10032171 | AF594 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-10032180 | AF647 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-10032181 | AF647 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-10032190 | AF680 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-10032191 | AF680 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100321A0 | AF700 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100321A1 | AF700 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100321B0 | AF750 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100321B1 | AF750 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100321C0 | APC Anti-human CD3 Antibody *UCHT1* | 25tests |
| AAT-100321C1 | APC Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100321C2 | APC Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100321D0 | APC/Cy7 Anti-human CD3 Antibody *UCHT1* | 25tests |
| AAT-100321D1 | APC/Cy7 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100321D2 | APC/Cy7 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100321E0 | APC/AF750 Anti-human CD3 Antibody *UCHT1* | 25tests |
| AAT-100321E1 | APC/AF750 Anti-human CD3 Antibody *UCHT1* | 100tests |
| AAT-100321E2 | APC/AF750 Anti-human CD3 Antibody *UCHT1* | 500tests |
| AAT-100321F0 | APC/iFluor™ 700 Anti-human CD3 Antibody *UCHT1* | 25tests |
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文献和实验一、 GFP背景概述 绿色荧光蛋白（green fluorescent protein, GFP）最早由Osamu Shimomura于1962年在水母（Aequorea victoria）（图2a）中发现。这种蛋白质在蓝色波长范围的光照激发下发出绿色荧光，其发光过程需要冷光蛋白质Aequorin的帮助，而且，这个冷光蛋白质可与钙离子（Ca2+）相互作用（图3）。在Aequorea victoria中发现的野生型绿色荧光蛋白的分子量较小，仅为27~30kDa，而编码GFP的基因序列也很短
5、其他应用 Cell surface organization in Natural Killer cells Dan Davis localization of calmodulin in S. pombe with GFP GFP-plakoglobin and expression plasmids Klymkowsky lab Molecular Motion Laboratory Yale University
领域奠基之作：Cell 重磅报道可胞外展示的糖基化修饰 RNA——glycoRNA
码 RNA 含有唾液酸化多糖，并且存在于多种细胞类型和哺乳动物物种体内。活细胞分析表明，大多数多糖修饰的 RNA 存在于细胞表面。 本研究通过对哺乳动物多糖修饰 RNA 的鉴定，揭示了 RNA 生物学和糖生物学之间存在直接的接口，并且 RNA 在细胞外生物学中的作用也得到了扩展，为转录后修饰调控 RNA 定位和功能提供了新的方向。 图片来源：Cell 研究内容 利用多糖代谢标志物标记细胞内 RNA 研究人员此前开发了基于代谢物标记和生物正交化学的方法
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