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- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
咨询
- 保质期：
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- 英文名：
2-Bromo-4-cyanothiazole
- 库存：
咨询
- 供应商：
无锡云萃生物
- CAS号：
848501-90-6
- 规格：
2mg/10mg/500mg/614g
| 规格： | 2mg | 产品价格： | ￥100.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥100.0 |
| 规格： | 500mg | 产品价格： | ￥100.0 |
| 规格： | 614g | 产品价格： | ￥214.0 |
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer
). However, for localization of cells containing transduced lacZ, chromogenic substrates that yield a precipitated product are desirable (Holt and O'Sullivan, 1958, Pearse 1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
immunoprecipitation protocol is based on about 1 x 107 cells (HEK293T or HeLa) with a transient transfection efficiency of 60-90% that was determined by fluorescence microscopy. (comment 1) Wash the cells 2 x with 5 ml PBS on ice; scrape cells off, transfer
