All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β - and γ -Actin are the principle constituents of filaments in other tissues
Isotype: IgG1 Immunogen: Full-length α-Actin protein of human. Tested applications: WB, IHC. Not yet tested in other applications. Recommended dilutions: WB: 1::1000—1:5000
IHC: 1:100—1:500
Optimal dilutions/concentrations should be determined by the end user. Form: Liquid Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol Storage: α-Actin antibody is stable for one year at -20°C. Avoid repeated freezing and thawing cycles.