ExoView外泌体全面表征试剂盒

仅需少量样品即可一次完成外泌体计数、粒径、蛋白表达、蛋白共定位、亚群分布的分析！

检测样本类型：对细胞培养上清、血浆、血清、尿液、脑脊液、唾液等生物样本中的外泌体直接进行分析。
捕获抗体种类：anti-CD81, anti-CD9, anti-CD63, 同型IgG对照；可自定义
单次上样体积：35 µl稀释样本
重复检测数目：3复孔
荧光抗体种类：CD9（Blue）/ CD81（Green）/ CD63（Red）
 

实验原理
① 35 μL外泌体样品滴加在芯片上孵育；
② 预先包被的抗体特异结合外泌体表面蛋白以捕获外泌体；
③ 再使用荧光抗体特异性标记需要表征的标记物；
④ 后用ExoView R100检测外泌体粒径、计数、蛋白表达（CD9，CD81，CD63等）及共定位。

检测流程

 

产品类别

产品货号	产品名称
EV-TETRA-C	人外泌体检测试剂盒
EV-TETRA-P	人血浆外泌体检测试剂盒
EV-TETRA-M2	鼠外泌体检测试剂盒
EV-TETRA-C-CAR	人外泌体内容物检测试剂盒
EV-TETRA-P-CAR	人血浆外泌体内容物检测试剂盒
EV-TC-FLEX	自由捕获人外泌体检测试剂盒
EV-TP-FLEX	自由捕获人血浆外泌体检测试剂盒
EV-TC-FLEX-CAR	自由捕获人外泌体内容物检测试剂盒
EV-TP-FLEX-CAR	自由捕获人血浆外泌体内容物检测试剂盒
EV-TM-FLEX	自由捕获鼠外泌体检测试剂盒
EV-TM-FLEX-CAR	自由捕获鼠外泌体内容物检测试剂盒
EV-FLEX-2	自由捕获外泌体检测试剂盒
EV-FLEX-2 -CAR	自由捕获外泌体内容物检测试剂盒
EV-CTETRA-1/2/3	人外泌体检测试剂盒+1/2/3个自定义捕获抗体
EV-CTETRA-1/2/3-CAR	人外泌体内容物检测试剂盒+1/2/3个自定义捕获抗体
EV-CUST-1/2/3/4/5/6	自定义1/2/3/4/5/6抗体捕获外泌体检测试剂盒
EV-CUST-1/2/3/4/5/6-CAR	自定义1/2/3/4/5/6抗体捕获外泌体内容物检测试剂盒

 

试剂盒特点

特异性捕获 芯片上可包被多达6种捕获抗体，特异性捕获含特定蛋白标记物的外泌体。
对阳性外泌体计数 芯片捕获外泌体后，可通过SP-IRIS技术直接检测样品中外泌体的数量。
单个外泌体蛋白共定位分析 检测每个外泌体的荧光信号并进行统计，可获得荧光共定位信息，用于分析样品中不同表型外泌体的比例（如右图所示）。
无需纯化 使用抗体捕获模式，防止样品中杂质影响结果，可直接检测血液、尿液和细胞培养液中的外泌体，未纯化样品的测量结果与纯化后基本一致（如右图所示）。
粒径分辨率高 高精度SP-IRIS技术，可检测≥50 nm的外泌体，测量结果与电子显微镜检测结果基本一致，并统计生成外泌体的粒径分布结果（如右图所示）。
可检测外泌体内容物 试剂盒配套相应的穿膜剂，可穿透外泌体并对外泌体内容物进行染色并检测，未穿膜时只能检测到跨膜蛋白CD9的荧光信号，穿膜后即可检测到外泌体内容物Syntenin的表达（如右图所示）。

DEMO报告

外泌体荧光数量统计

外泌体粒径检测

 

荧光强度与粒径关系

 

荧光共定位分析

发表文章

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