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- 库存：
大量
- 英文名：
Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
1 kit
齐源生物专业代理美国AAT Bioquest产品，并有大量储存现货欢迎致电
关键词：Buccutite 过氧化物酶（HRP）抗体偶联试剂盒 适合标记1mg蛋白详细如下
Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins. One end of the crosslinker reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the other end reacts (via maleimide) with the thiol groups (-SH) found in the amino acid cysteine. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. In addition it is quite difficult and tedious to quantify the number of maleimide groups on a protein. Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit is designed for preparing horseradish peroxidase (HRP) conjugates directly from proteins, peptides, and other ligands that contain a free amino group. The HRP provided in our kit has been pre-activated with our proprietary linker Buccutite™ FOL, and can be directly used for conjugation. The entire process only requires two simple mixings without further purification required. The Buccutite™ FOL-activated HRP readily reacts with Buccutite™ MTA-containing molecules under extremely mild neutral conditions without any catalyst required. Compared to commonly used SMCC and other similar technologies, our Buccutite™ bioconjugation system is much more robust and easier to use. It enables faster and quantitative conjugation of biomolecules with higher efficiencies and yields.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
关键词：Buccutite 过氧化物酶（HRP）抗体偶联试剂盒 适合标记1mg蛋白详细如下
Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins. One end of the crosslinker reacts (via NHS ester) with amines (-NH2) found in the amino acid lysine and N-terminus, and the other end reacts (via maleimide) with the thiol groups (-SH) found in the amino acid cysteine. However, SMCC-modified protein is extremely unstable and often self-reactive since proteins often contain both amine and thiol groups that cause significant amount of homo-crosslinking. In addition it is quite difficult and tedious to quantify the number of maleimide groups on a protein. Buccutite™ Peroxidase (HRP) Antibody Conjugation Kit is designed for preparing horseradish peroxidase (HRP) conjugates directly from proteins, peptides, and other ligands that contain a free amino group. The HRP provided in our kit has been pre-activated with our proprietary linker Buccutite™ FOL, and can be directly used for conjugation. The entire process only requires two simple mixings without further purification required. The Buccutite™ FOL-activated HRP readily reacts with Buccutite™ MTA-containing molecules under extremely mild neutral conditions without any catalyst required. Compared to commonly used SMCC and other similar technologies, our Buccutite™ bioconjugation system is much more robust and easier to use. It enables faster and quantitative conjugation of biomolecules with higher efficiencies and yields.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-104100S0 | iFluor™ A7 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-104100S1 | iFluor™ A7 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-104100T0 | mFluor™ Blue 570 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-104100T1 | mFluor™ Blue 570 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-104100U0 | mFluor™ Green 620 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-104100U1 | mFluor™ Green 620 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-104100V0 | mFluor™ Red 700 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-104100V1 | mFluor™ Red 700 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-104100W0 | mFluor™ Red 780 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-104100W1 | mFluor™ Red 780 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-104100X0 | mFluor™ UV375 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-104100X1 | mFluor™ UV375 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-104100Y0 | mFluor™ UV460 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-104100Y1 | mFluor™ UV460 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-104100Z0 | mFluor™ Violet 450 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-104100Z1 | mFluor™ Violet 450 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-10410100 | mFluor™ Violet 500 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-10410101 | mFluor™ Violet 500 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-10410110 | mFluor™ Violet 510 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-10410111 | mFluor™ Violet 510 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-10410120 | mFluor™ Violet 540 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-10410121 | mFluor™ Violet 540 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-10410140 | AF350 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-10410141 | AF350 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-10410150 | AF488 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-10410151 | AF488 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-10410160 | AF555 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-10410161 | AF555 Anti-human CD41 Antibody *HIP8* | 500tests |
| AAT-10410170 | AF594 Anti-human CD41 Antibody *HIP8* | 100tests |
| AAT-10410171 | AF594 Anti-human CD41 Antibody *HIP8* | 500tests |
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文献和实验相关实验
差异。如以2,2’-联氮-双-[3-乙基苯并噻唑啉]-6-磺酸(ABTS)为氢供体，则只能保护20％的酶活性，而以邻联茴香胺(ODA)为氢供体时，酶活性的保护提高至90％。HRP之所以是迄今为止在ELISA中应用最为广泛的标记用酶，主要是因为其一方面易于提取，价格相对低廉；另一方面性质稳定，耐热及有机溶剂的作用，与抗原或抗体偶联后，活性很少受损失。
组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
用了许多方法检测小鼠的肝脏、脑部、睾丸等组织的细胞凋亡，其中 TUNEL 法做的最多，我购买的试剂盒主要是 roche、 promega 公司，结果大多数成功，偶尔也有失败，下面我把实验中的关键问题与大家共享一下，同时也希望能对初学者有所帮忙。 TUNEL 法的实验原理 基本原理：对不同组织切片先增加细胞膜通透性，然后让 rTDT 和生物标记的 dTUTP 进入细胞内，在 rTDT 的辅助下 dTUTP 与核断裂的 DNA 3』-OH 结合，再用 HRP 标记的链霉
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Buccutite 过氧化物酶（HRP）抗体偶联试剂盒 适合标记1mg蛋白
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